Effects of smokeless nicotine on blood physiology, biochemical, and histological alterations using Labeo rohita as a model organism

Authors

  • Shabbir Ahmad Department of Zoology, Faculty of Life Sciences, University of Okara, Okara, Pakistan
  • Hasnain Akmal Department of Zoology, Faculty of Life Sciences, University of Okara, Okara, Pakistan
  • Sajid Ali Department of Zoology, Faculty of Life Sciences, University of Okara, Okara, Pakistan
  • Kamran Jafar Department of Zoology, Faculty of Life Sciences, University of Okara, Okara, Pakistan
  • Muhammad Shoaib Department of Zoology, Faculty of Life Sciences, University of Okara, Okara, Pakistan
  • Muqadas Shahzadi Department of Zoology, Faculty of Life Sciences, University of Okara, Okara, Pakistan
  • Iqra Akram Department of Zoology, Faculty of Life Sciences, University of Okara, Okara, Pakistan
  • Taqi Shahid Jaffari Department of Zoology, Faculty of Life Sciences, University of Okara, Okara, Pakistan
  • Irfan Ahmad Department of Zoology, Faculty of Life Sciences, University of Okara, Okara, Pakistan
  • Arva Mehmood Department of Zoology, Faculty of Life Sciences, University of Okara, Okara, Pakistan
  • Khurram Shahzad Department of Zoology, Faculty of Life Sciences, University of Okara, Okara, Pakistan

Keywords:

Nicotine; biochemistry; lipid profile; hematology; histology; Labeo rohita

Abstract

Objective: The present research was conducted to evaluate the negative effects of nicotine powder on the blood physiology, and biochemical and histological alterations of Labeo rohita. Materials and Methods: Fish were divided into four groups (1–4). Fish groups 2, 3, and 4 were exposed to different concentrations of nicotine, such as 0.75, 1.25, and 1.75 mg/l, while group 1 acted as a control. To find out the long-term impact of nicotine on body physiology, we conducted a 42-day experiment. After the completion of the experiment, hematology, biochemical assays, and histology were done. Results: Results revealed a considerable increase in HGB, red blood cells, WBCs, hematocrit, mean corpuscular volume, red cell distribution width -SD, procalcitonin, neutrophils, lymphocytes, monocytes, triglycerides, total cholesterol, low-density lipoprotein, very low-density lipoprotein, alanine transaminase, aspartate aminotransferase, globulin, thyroid stimulating hormone, BUN, creatinine, and blood glucose levels, whereas mean corpuscular hemoglobin concentration, mean corpuscular hemoglobin, RDW, platelet, high-density lipoprotein, albumin, total proteins, and T3 levels were significantly (p ≤ 0.05) decreased in exposed fish as compared to control group fish. Histological alterations showed that exposure to smokeless nicotine causes deleterious and degenerative effects in the liver, kidney, and gills of exposed fish. Conclusion: Nicotine administration in fish results in adverse effects on different biochemical and hematological parameters and causes histological alterations in some vital organs of exposed fish.

Adv. Vet. Anim. Res., 11(2): 463-473, June 2024

http://doi.org/10.5455/javar.2024.k796

Downloads

Download data is not yet available.
Abstract
41
PDF
56

Downloads

Published

2024-06-19

How to Cite

Ahmad, S., Akmal, H., Ali, S., Jafar, K., Shoaib, M., Shahzadi, M., Akram, I., Jaffari, T. S., Ahmad, I., Mehmood, A., & Shahzad, K. (2024). Effects of smokeless nicotine on blood physiology, biochemical, and histological alterations using Labeo rohita as a model organism. Journal of Advanced Veterinary and Animal Research, 11(2), 463–473. Retrieved from https://banglajol.info/index.php/JAVAR/article/view/75740

Issue

Vol. 11 No. 2 (2024)

Section

Original Articles

License

Copyright (c) 2024 Shabbir Ahmad

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Authors who publish with this journal agree to the following terms:

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).