Effects of smokeless nicotine on blood physiology, biochemical, and histological alterations using Labeo rohita as a model organism

Authors

  • Shabbir Ahmad Department of Zoology, Faculty of Life Sciences, University of Okara, Okara, Pakistan
  • Hasnain Akmal Department of Zoology, Faculty of Life Sciences, University of Okara, Okara, Pakistan
  • Sajid Ali Department of Zoology, Faculty of Life Sciences, University of Okara, Okara, Pakistan
  • Kamran Jafar Department of Zoology, Faculty of Life Sciences, University of Okara, Okara, Pakistan
  • Muhammad Shoaib Department of Zoology, Faculty of Life Sciences, University of Okara, Okara, Pakistan
  • Muqadas Shahzadi Department of Zoology, Faculty of Life Sciences, University of Okara, Okara, Pakistan
  • Iqra Akram Department of Zoology, Faculty of Life Sciences, University of Okara, Okara, Pakistan
  • Taqi Shahid Jaffari Department of Zoology, Faculty of Life Sciences, University of Okara, Okara, Pakistan
  • Irfan Ahmad Department of Zoology, Faculty of Life Sciences, University of Okara, Okara, Pakistan
  • Arva Mehmood Department of Zoology, Faculty of Life Sciences, University of Okara, Okara, Pakistan
  • Khurram Shahzad Department of Zoology, Faculty of Life Sciences, University of Okara, Okara, Pakistan

Keywords:

Nicotine; biochemistry; lipid profile; hematology; histology; Labeo rohita

Abstract

Objective: The present research was conducted to evaluate the negative effects of nicotine powder on the blood physiology, and biochemical and histological alterations of Labeo rohita. Materials and Methods: Fish were divided into four groups (1–4). Fish groups 2, 3, and 4 were exposed to different concentrations of nicotine, such as 0.75, 1.25, and 1.75 mg/l, while group 1 acted as a control. To find out the long-term impact of nicotine on body physiology, we conducted a 42-day experiment. After the completion of the experiment, hematology, biochemical assays, and histology were done. Results: Results revealed a considerable increase in HGB, red blood cells, WBCs, hematocrit, mean corpuscular volume, red cell distribution width -SD, procalcitonin, neutrophils, lymphocytes, monocytes, triglycerides, total cholesterol, low-density lipoprotein, very low-density lipoprotein, alanine transaminase, aspartate aminotransferase, globulin, thyroid stimulating hormone, BUN, creatinine, and blood glucose levels, whereas mean corpuscular hemoglobin concentration, mean corpuscular hemoglobin, RDW, platelet, high-density lipoprotein, albumin, total proteins, and T3 levels were significantly (p ≤ 0.05) decreased in exposed fish as compared to control group fish. Histological alterations showed that exposure to smokeless nicotine causes deleterious and degenerative effects in the liver, kidney, and gills of exposed fish. Conclusion: Nicotine administration in fish results in adverse effects on different biochemical and hematological parameters and causes histological alterations in some vital organs of exposed fish.

Adv. Vet. Anim. Res., 11(2): 463-473, June 2024

http://doi.org/10.5455/javar.2024.k796

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Published

2024-06-19

How to Cite

Ahmad, S., Akmal, H., Ali, S., Jafar, K., Shoaib, M., Shahzadi, M., Akram, I., Jaffari, T. S., Ahmad, I., Mehmood, A., & Shahzad, K. (2024). Effects of smokeless nicotine on blood physiology, biochemical, and histological alterations using Labeo rohita as a model organism. Journal of Advanced Veterinary and Animal Research, 11(2), 463–473. Retrieved from https://banglajol.info/index.php/JAVAR/article/view/75740

Issue

Vol. 11 No. 2 (2024)

Section

Original Articles

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Copyright (c) 2024 Shabbir Ahmad

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