Regulatory effect of apoptosis on morphological changes in cell mass of porcine blastocyst through supplementation of rapamycin during in vitro culture

Authors

  • Dae Seung Kim Major in the Animal Biotechnology, Graduate School of Future Convergence Technology, Hankyong National University, Anseong, Korea.
  • Sang Hwan Kim Institute of Genetic Engineering, Hankyong National University, Ansung, Korea.
  • Jong Taek Yoon Department of Animal Life and Environment Science, Hankyong National University, Ansung, Korea.

Keywords:

MMPs; apoptosis; rapamycin; IVF; porcine

Abstract

Objective: The study of an in vitro embryosis is crucial in genetics for breed improvement and reproduction in livestock, identifying the causes of infertility, and stem cell application. Meanwhile, the problem of nucleic acid denaturation observed during embryo development is yet to be resolved. This study was set out to analyze the nucleic acid denaturation during the development of in vitro embryos.  Materials and Methods: Using an in-vitro fertilization-embryo in porcine, the cell development and apoptosis were evaluated by adding rapamycin by concentration to the TCM-199 containing 10% FBS or 10% porcine follicle fluid (pFF). Real-time PCR, zymography, DNA fragment, Western blot, and immunofluorescence analysis were also carried out to determine the development rate of inner cell mass in the in-vitro fertilization-embryo.  Results: The findings indicated that the addition of rapamycin to the 10% pFF group during in vitro maturation led to an increase in the rates of cleavage and blastocyst development and the expres­sion of active matrix metallopeptidase (MMP-9), while nucleic acid denaturation was suppressed. In other words, the addition of rapamycin was found to increase the expression of MMP-2 in the inner cell mass and trophoblast, while it inhibited apoptosis.  Conclusion: The addition of rapamycin influences the regulation of apoptosis and MMPs, and based on this, it is presumed to have a positive effect on blastocyst development.

J. Adv. Vet. Anim. Res., 7(4): 614-620, Dec 2020

http://doi.org/10.5455/javar.2020.g459

Downloads

Download data is not yet available.
Abstract
25
PDF
23

Downloads

Published

2020-10-01

How to Cite

Kim, D. S., Kim, S. H., & Yoon, J. T. (2020). Regulatory effect of apoptosis on morphological changes in cell mass of porcine blastocyst through supplementation of rapamycin during in vitro culture. Journal of Advanced Veterinary and Animal Research, 7(4), 614–620. Retrieved from https://banglajol.info/index.php/JAVAR/article/view/75771

Issue

Vol. 7 No. 4 (2020)

Section

Original Articles

License

Copyright (c) 2020 Dae Seung Kim, Sang Hwan Kim, Jong Taek Yoon

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Authors who publish with this journal agree to the following terms:

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).