Isolation and characterization of strictly anaerobic cellulolytic rumen bacterial species from Sahiwal cattle

Authors

  • Muhammad Ashiqul Alam Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh
  • Md Jannat Hossain Department of Microbiology and Public Health, Khulna Agricultural University, Khulna, Bangladesh
  • M Sohidullah Department of Microbiology and Public Health, Khulna Agricultural University, Khulna, Bangladesh
  • Md Shahidur Rahman Khan Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh
  • Khan Md Shaiful Islam Department of Animal Nutrition, Bangladesh Agricultural University, Mymensingh, Bangladesh

Keywords:

Bacteria; cattle; cellulolytic; rumen; Ruminococcus spp

Abstract

Objective: To isolate and characterize cellulolytic rumen bacteria from the rumen of Sahiwal cattle using rumen bacterial inoculum. Materials and Methods: The ruminal liquid was kept at an optimal pH of 6.9 and a redox potential of less than −300 mV while being incubated anaerobically at 39°C in a medium containing rumen fluid glucose cellobiose agar. By using the Hungate technique, the organisms were detected based on their morphological, physiological, biochemical, and molecular testing. Results: The findings revealed that the isolated Ruminococcus albus, and Ruminococcus flavifaciens were obligate anaerobic, generally Gram-positive, nonmotile cocci or rod, single or pair, occasionally short chain, producing yellow pigment when grown on cellulose, and having a clear zone around the colonies. Both isolate fermented sugars such as cellobiose, glucose, and lactose, as well as decomposed xylan. The results also showed that the isolates recognized as Ruminococcus spp., a cellulolytic rumen bacterium, were catalase-negative, indole-negative, and gelatin liquefaction-positive. Conclusion: Isolation and characterization of Ruminococcus spp. may be helpful for Bangladesh in reducing the cost of producing poultry feed and circumventing restrictions on rice bran use. We can also develop more efficient and long-lasting plans to enhance poultry performance and feed efficiency, as well as increase the nutritional value of rice bran used as broiler feed, by understanding how various Ruminococcus spp. function in this process.

Adv. Vet. Anim. Res., 11(1): 1-8, March 2024

http://doi.org/10.5455/javar.2024.k740

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Published

2024-03-07

How to Cite

Alam, M. A., Hossain, M. J., Sohidullah, M., Khan, M. S. R., & Islam, K. M. S. (2024). Isolation and characterization of strictly anaerobic cellulolytic rumen bacterial species from Sahiwal cattle. Journal of Advanced Veterinary and Animal Research, 11(1), 1–8. Retrieved from https://banglajol.info/index.php/JAVAR/article/view/75889

Issue

Vol. 11 No. 1 (2024)

Section

Original Articles

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Copyright (c) 2024 Muhammad Ashiqul Alam

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