Determination of total phenolics, flavonoids, and testing of antioxidant and antibacterial activities of red ginger (Zingiber officinale var. Rubrum)

Authors

  • Ucop Haroen Faculty of Animal Science, Jambi University, Sumatra, Indonesia
  • Syafwan Syafwan Faculty of Animal Science, Jambi University, Sumatra, Indonesia
  • Kiki Kurniawan Research Center for Vaccine and Drugs Development, National Research and Innovation Agency, Cibinong, Indonesia
  • Agus Budiansyah Faculty of Animal Science, Jambi University, Sumatra, Indonesia

Keywords:

Zingiber officinale var. Rubrum; antioxidant; antibacterial; MIC; MBC

Abstract

Objective: The purpose of this study was to select the active fraction of red ginger (Zingiber officinale var. Rubrum) for its antioxidant and antibacterial activities against Staphylococcus aureus (AMC 6934), Bacillus subtilis (AMC 7923), Pseudomonas aeruginosa (AMC 8973), and Escherichia coli (AMC 5761). Materials and Methods: A total of 2 kg of dry red ginger rhizome powder was macerated in stages with different levels of solvent polarity to extract the chemical composition within the red ginger powder sample. The extraction process begins with a non-polar solvent (n-hexane) by soaking the red ginger powder sample for 3 × 24 h. Results: The red ginger extract fractionated with methanol produced alkaloids, phenolics, flavonoids, and coumarins, while the fractionation using n-hexane produced alkaloids and triterpenoids only. The fractionation with ethyl acetate produced alkaloids, phenolics, flavonoids, triterpenoids, saponins, and coumarins. The antioxidant activity test was 49.261 mg/l for the ethyl acetate fraction, 146.648 mg/l for the methanol fraction, and 300.865 mg/l for the n-hexane fraction. Conclusion: The ethyl acetate fraction was effectively powerful in inhibiting the growth of Grampositive and Gram-negative bacteria. All fractions had moderate antibacterial activity; however, the performance of ethyl acetate in the red ginger extract was better than that of methanol and n-hexane.

Adv. Vet. Anim. Res., 11(1): 114-124, March 2024

http://doi.org/10.5455/javar.2024.k755

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Published

2024-03-19

How to Cite

Haroen, U., Syafwan, S., Kurniawan, K., & Budiansyah, A. (2024). Determination of total phenolics, flavonoids, and testing of antioxidant and antibacterial activities of red ginger (Zingiber officinale var. Rubrum). Journal of Advanced Veterinary and Animal Research, 11(1), 114–124. Retrieved from https://banglajol.info/index.php/JAVAR/article/view/75987

Issue

Vol. 11 No. 1 (2024)

Section

Original Articles

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Copyright (c) 2024 Ucop Haroen

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