Effective isolation of Sertoli cells from New Zealand rabbit testis

Authors

  • Wen Qian Zhu Animal Histology and Embryology, College of Veterinary Medicine, Jilin University, Changchun, Jilin Province, China.
  • De Cai Yang Animal Histology and Embryology, College of Veterinary Medicine, Jilin University, Changchun, Jilin Province, China.
  • Yu Jiang Animal Histology and Embryology, College of Veterinary Medicine, Jilin University, Changchun, Jilin Province, China.
  • Ning Ning Cai Animal Histology and Embryology, College of Veterinary Medicine, Jilin University, Changchun, Jilin Province, China.
  • Rui Yang Animal Histology and Embryology, College of Veterinary Medicine, Jilin University, Changchun, Jilin Province, China.
  • Xue Ming Zhang Animal Histology and Embryology, College of Veterinary Medicine, Jilin University, Changchun, Jilin Province, China.

Keywords:

Enrichment; identification; rabbit; separation; Sertoli cells

Abstract

Objective: Sertoli cells (SCs) are important sustentacular cells in the seminiferous tubules of the testis. Isolation and identification of SCs are the premise for studying their functions. Since New Zealand rabbit is a stable strain which is widely used for biomedical research and animal farming, this study aimed to develop a simple and effective protocol for SC isolation in New Zealand rabbits.  Materials and Methods: The SCs of three 30-day-old New Zealand rabbits were isolated by incu­bation with enzymatic digestion I (Dulbecco’s modified Eagle medium supplemented with 1 mg/ ml collagenase IV and 50 μg/ml DNase I) and digestion II (digestion I + 1 mg/ml hyaluronidase + 1 mg/ml trypsin), as well as differential plating. The cells were enriched and identified by using immunocytochemical staining and reverse transcription polymerase chain reaction analysis.  Results: Homogeneous cells were obtained. They presented the typical large cell body and an irregular pyramidal shape after differential plating and passaging. These cells expressed mRNA of the SC marker sex-determining region Y-box 9 (SOX9) instead of the Leydig cell marker StAR. Immunocytochemically, they are positive of SOX9, GATA binding protein 4, and androgen-binding protein.  Conclusion: The SCs were enriched from the testicular tissues of prepubertal New Zealand rabbits by a simple and effective protocol, which provides a basis for further theoretical researches and practical applications.

J. Adv. Vet. Anim. Res., 8(2): 218-223, June 2021

Downloads

Download data is not yet available.
Abstract
21
PDF
16

Downloads

Published

2021-06-18

How to Cite

Zhu, W. Q., Yang, D. C., Jiang, Y., Cai, N. N., Yang, R., & Zhang, X. M. (2021). Effective isolation of Sertoli cells from New Zealand rabbit testis. Journal of Advanced Veterinary and Animal Research, 8(2), 218–223. Retrieved from https://banglajol.info/index.php/JAVAR/article/view/76249

Issue

Vol. 8 No. 2 (2021)

Section

Original Articles

License

Copyright (c) 2021 Wen Qian Zhu, De Cai Yang, Yu Jiang, Ning Ning Cai, Rui Yang, Xue Ming Zhang

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Authors who publish with this journal agree to the following terms:

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).