Bioinformatics analysis of structures and ligand-bindings of predicted zymogen granule protein observed on Bali cattle (Bos javanicus) saliva

Authors

  • Djoko Kisworo Faculty of Animal Science, University of Mataram, Mataram, Indonesia.
  • Sulaiman Ngongu Depamede Faculty of Animal Science, University of Mataram, Mataram, Indonesia.

Keywords:

Mannose-binding lectin; innate immunity; signal peptide; amino acid; sequence; MALDI-TOF/TOF-MS

Abstract

Objective: Previously, we have shown that predicted zymogen granule protein 16 homolog B (P-G3MZ19) existed in Bali cattle (Bos javanicus) saliva. It was suggested that P-G3MZ19 is a mem­ber of the mannose-binding lectin family that plays an essential role in innate immunity. In the present study, we aimed to analyze the structure and ligand-binding of P-3MZ19 in Bali cattle saliva.  Materials and Methods: Saliva of four adult healthy Bali cattle was collected, lyophilized, and subjected to two-dimensional (2-D) gel electrophoresis. The target spot of around 17 kDa related to P-G3MZ19 was excised for matrix-assisted laser desorption ionization time-of-flight mass spec­trometer/time-of-flight mass spectrometer mass spectrometry analysis and sequencing. The structure and the ligand-binding of P-3MZ19 were analyzed using bioinformatics software pro­grams published elsewhere.  Results: Based on Iterative Threading ASSEmbly Refinement the 3D model of P-G3MZ19 was suggested to have similarities to exo-alpha-sialidase (EC 3.2.1.18); while its ligand-binding sites consisted of seven residues, i.e., 25aa-26aa (Gly-Gly), 95aa (Phe), 138aa (Tyr), 140aa (Leu), 141aa (Gly), and 143aa (Thr).  Conclusion: The structure of P-G3MZ19 of Bali cattle saliva and its ligand-binding sites have been successfully determined by using bioinformatics techniques. The biological and immunological roles of the peptide are currently under investigation based on P-G3MZ19 synthetic peptides.

J. Adv. Vet. Anim. Res., 8(2): 224-229, June 2021

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Published

2021-06-18

How to Cite

Kisworo, D., & Depamede, S. N. (2021). Bioinformatics analysis of structures and ligand-bindings of predicted zymogen granule protein observed on Bali cattle (Bos javanicus) saliva. Journal of Advanced Veterinary and Animal Research, 8(2), 224–229. Retrieved from https://banglajol.info/index.php/JAVAR/article/view/76250

Issue

Vol. 8 No. 2 (2021)

Section

Original Articles

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Copyright (c) 2021 Djoko Kisworo, Sulaiman Ngongu Depamede

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