Antibiotic resistance patterns of Staphylococcus spp. isolated from fast foods sold in different restaurants of Mymensingh, Bangladesh

Authors

  • Monami Rahman Urmi Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.
  • Wahedul Karim Ansari Department of Microbiology and Public Health, Faculty of Animal Science and Veterinary Medicine, Patuakhali Science and Technology University, Barisal, Bangladesh.
  • Md Saiful Islam Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.
  • Md Abdus Sobur Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.
  • Marzia Rahman Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.
  • Md Tanvir Rahman Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

Keywords:

Antibiotic resistance; coagulasepositive; fast foods; MAR; MDR; public health; Staphylococcus aureus

Abstract

Objective: Fast foods are often responsible for staphylococcal foodborne illness. The present study was carried out to isolate Staphylococcus spp. from various fast foods sold in Mymensingh and to determine their antibiogram.  Materials and Methods: Overall, 60 samples of fast foods sold in different restaurants were screened by culture, biochemical tests, and polymerase chain reaction (PCR) to isolate and iden­tify Staphylococcus spp., followed by employing of disk diffusion method to determine their anti­biotic resistance patterns.  Results: Among these 60 samples, 8 [13.33%, 95% confidence interval (CI): 6.91%–24.17%] were positive for Staphylococcus spp. by cultural and biochemical properties. By PCR, four (6.67%, 95% CI: 2.62%–15.93%) isolates were confirmed as Staphylococcus aureus by targeting nuc gene. Additionally, all the S. aureus isolates were coagulase-positive. By antibiogram profiles, all the Staphylococcus isolates exhibited resistance to azithromycin and erythromycin (95% CI: 67.56%– 100.00%), and frequently resistance to cefixime (75%, 95% CI: 40.93%–95.56%), ampicillin (50%, 95% CI: 21.52%–78.48%), and amoxicillin (37.5%, 95% CI: 13.68%–69.43%); moderate to lower resistance was found against cefotaxime, gentamicin, and doxycycline. In addition, all the isolates were sensitive to ciprofloxacin and chloramphenicol. Interestingly, 75% (6/8; 95% CI: 40.93%– 95.56%) isolates were multidrug-resistant (MDR) in nature. Furthermore, the indices of multiple antibiotic resistance (MAR) were ranged from 0.2 to 0.6.  Conclusion: This study revealed that fast foods sold in restaurants were contaminated with MDR and MAR Staphylococcus isolates, having potential public health significance.

J. Adv. Vet. Anim. Res., 8(2): 274-281, June 2021

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Published

2021-06-23

How to Cite

Urmi, M. R., Ansari, W. K., Islam, M. S., Sobur, M. A., Rahman, M., & Rahman, M. T. (2021). Antibiotic resistance patterns of Staphylococcus spp. isolated from fast foods sold in different restaurants of Mymensingh, Bangladesh. Journal of Advanced Veterinary and Animal Research, 8(2), 274–281. Retrieved from https://banglajol.info/index.php/JAVAR/article/view/76261

Issue

Vol. 8 No. 2 (2021)

Section

Original Articles

License

Copyright (c) 2021 Monami Rahman Urmi, Wahedul Karim Ansari, Md Saiful Islam, Md Abdus Sobur, Marzia Rahman, Md Tanvir Rahman

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