Clinicopathological and bacteriological studies on lamb bacterial enteritis and monitoring the oregano oil and vitamins A,D3,E effect on its treatment

Authors

  • Asmaa Abdallah Darwish Department of animal and poultry health, animal and poultry division, Desert Research Center, Cairo, Egypt.
  • Marwa Fawzy Department of animal and poultry health, animal and poultry division, Desert Research Center, Cairo, Egypt.
  • Wafaa Abd Latif Osman Department of animal and poultry health, animal and poultry division, Desert Research Center, Cairo, Egypt.
  • Eman A El Ebissy Department of animal and poultry health, animal and poultry division, Desert Research Center, Cairo, Egypt.

Keywords:

Bacterial lamb enteritis; clinicopahtological parameters; oregano oil extract 15%; vitamins A,D3,E; 16SrRNA

Abstract

Objective: The objective of the study was to assess the effect of A,D3,E (I/M) and oregano oil extract 15% on some clinicopathological parameters during lamb bacterial enteritis treatment.  Materials and Methods: Sixty Barki lambs, 20 apparently healthy (control group) and, 40 suf­fered from bacterial enteritis [enteric group (EG)], were subdivided into four treated groups (TGs): antibiotic group (AG), antibiotic + A,D3,E group (A + A,D3,E), antibiotic + oregano oil (AOG), and oregano group (OG). Fecal swabs were collected from EG then aseptically cultured, isolated, phe­notypically identified, genotypically confirmed, and sequenced by PCR 16srRNA. Paper disk dif­fusion test was used for estimation of oregano oil extract 15% antibacterial activity. After blood sample aspiration from all animals, they were clinicopathologically and statistically analyzed.  Results: Escherichia coli, followed by Salmonella species and then Klebsiella species, was the main causative agents of lamb diarrhea and were susceptible to oregano oil extract 15%. A + A,D3,E and AOG showed significant (p < 0.05) enhancement of some clinicopathological parameters more than AG or OG. Matrix metalloproteinases (MMP-2 and MMP-9) and total antioxidant capacity (TAC), yielded area under the curve, sensitivity, negative predictive value as 1, 100% and 100% respectively, were determined in both EG and TGs.  Conclusion: Oregano oil extract 15% has good antibacterial properties against enteric bacteria in vitro and in vivo. The combination between antibiotic and antioxidant vitamins or oregano plant extract of 15% has a good impact on some clinicopathological alterations in lamb bacterial enteri­tis treatment. TAC, MMP-9, and MMP-2 may be good markers for the disease and its treatment follow-up.

J. Adv. Vet. Anim. Res., 8(2): 291-299, June 2021

Downloads

Download data is not yet available.
Abstract
14
PDF
12

Downloads

Published

2021-06-23

How to Cite

Darwish, A. A., Fawzy, M., Osman, W. A. L., & Ebissy, E. A. E. (2021). Clinicopathological and bacteriological studies on lamb bacterial enteritis and monitoring the oregano oil and vitamins A,D3,E effect on its treatment. Journal of Advanced Veterinary and Animal Research, 8(2), 291–299. Retrieved from https://banglajol.info/index.php/JAVAR/article/view/76265

Issue

Vol. 8 No. 2 (2021)

Section

Original Articles

License

Copyright (c) 2021 Asmaa Abdallah Darwish, Marwa Fawzy, Wafaa Abd Latif Osman, Eman A El Ebissy

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Authors who publish with this journal agree to the following terms:

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).