ITS1-PCR based identification of chicken Eimeria species in poultry litter from Mymensingh district, Bangladesh

Authors

  • Mohammad Zahangir Alam Department of Parasitology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh.
  • Anita Rani Dey Department of Parasitology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh.
  • Shanaz Parvin Department of Parasitology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh.
  • Shirin Akter Department of Parasitology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh.
  • Sharmin Aqter Rony Department of Parasitology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh.

Keywords:

Coccidiosis; Eimeria species; identification; ITS1-PCR; chicken

Abstract

Objectives: The purpose of this study was to determine the species composition of Eimeria circu­lating in Mymensingh district, Bangladesh, using Internal Transcribed Spacer 1 (ITS1) sequences in polymerase chain reaction (PCR) assay.  Materials and Methods: Coccidian oocysts were isolated and sporulated in a solution containing 2% potassium dichromate from litter slurry collected from 13 commercially active broiler farms in the research region. Genomic DNA was isolated from sporulated oocysts and used to amplify the Eimeria species-specific ITS1 gene by PCR amplification. Electrophoresis of 1.5% agarose gel was used to visualize the amplified PCR products.  Results: In the study samples from Mymensingh district, Bangladesh, the presence of Eimeria brunetti, Eimeria acervulina, Eimeria necatrix, Eimeria mitis, and Eimeria tenella was identified.  Conclusions: The findings of this study may shed light on the zonal approach to chicken coccidio­sis control. Additionally, it suggests that ITS1-based PCR might be used in the field to accurately identify Eimeria species.

J. Adv. Vet. Anim. Res., 8(3): 489-493, September 2021

http://doi.org/10.5455/javar.2021.h538

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Published

2021-09-20

How to Cite

Alam, M. Z., Dey, A. R., Parvin, S., Akter, S., & Rony, S. A. (2021). ITS1-PCR based identification of chicken Eimeria species in poultry litter from Mymensingh district, Bangladesh. Journal of Advanced Veterinary and Animal Research, 8(3), 489–493. Retrieved from https://banglajol.info/index.php/JAVAR/article/view/76914

Issue

Vol. 8 No. 3 (2021)

Section

Short Communications

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Copyright (c) 2021 Mohammad Zahangir Alam, Anita Rani Dey, Shanaz Parvin, Shirin Akter, Sharmin Aqter Rony

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