Multidrug-resistant and enterotoxigenic methicillin-resistant Staphylococcus aureus isolated from raw milk of cows at small-scale production units

Authors

  • Hamza Mohamed Eid Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt. https://orcid.org/0000-0002-8415-2014
  • Heba Sayed El Mahallawy Department of Animal Hygiene, Zoonoses, and Animal Behaviour and Management, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt. https://orcid.org/0000-0001-6468-2483
  • Sahar Roshdi Mohammed Head Research of Bacteriology and Chief of Bacteriology Department, Animal Health Research Institute, Dokki, Egypt.
  • Nehal Ezzat Yousef Mohammed Food Inspector, Animal Health Research Institute, Damietta, Egypt.
  • Nada Hussein Eidaroos Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt.

Keywords:

Antimicrobial resistance; cow’s milk; enterotoxin genes; MRSA; Staphylococcus aureus; virulence genes

Abstract

Objective: Staphylococcus aureus (S. aureus) has evolved as one of the most significant bacteria causing food poisoning outbreaks worldwide. This study was carried out to investigate the prevalence, antibiotic sensitivity, virulence, and enterotoxin production of S. aureus in raw milk of cow from small-scale production units and house-raised animals in Damietta governorate, Egypt. Material and Methods: The samples were examined bacteriologically, and antimicrobial sensitivity testing was carried out. Moreover, isolates were characterized by the molecular detection of antimicrobial resistance, virulence, and enterotoxin genes. Results: Out of 300 milk samples examined, S. aureus was isolated from 50 samples (16.7%). Antibiotic sensitivity testing revealed that isolates were resistant to β-lactams (32%), tetracycline (16%), and norfloxacin (16%); however, they showed considerable sensitivity to ceftaroline and amikacin (72%). Multidrug-resistance (MDR) has been observed in eight isolates (16%), with a MDR index (0.5) in all of them. Of the total S. aureus isolates obtained, methicillin-resistant S. aureus (MRSA) has been confirmed molecularly in 16/50 (32%) and was found to carry mecA and coa genes, while virulence genes; hlg (11/16, 68.75%) and tsst (6/16, 37.5%) were amplified at a lower percentage, and they showed a significant moderate negative correlation (r = −0.59, p-value > 0.05). Antibiotic resistance genes have been detected in resistant isolates relevant to their phenotypic resistance: blaZ (100%), tetK (50%), and norA (50%). Fifty percent of MRSA isolates carried the seb enterotoxin gene. Conclusion: High detection rate of MRSA and MDR isolates from milk necessitates the prompt implementation of efficient antimicrobial stewardship guidelines, especially at neglected smallscale production units.

J. Adv. Vet. Anim. Res., 9(1): 113–121, March 2022

http://doi.org/10.5455/javar.2022.i575

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Published

2022-03-30

How to Cite

Eid, H. M., El Mahallawy, H. S., Mohammed, S. R., Mohammed, N. E. Y., & Eidaroos, N. H. (2022). Multidrug-resistant and enterotoxigenic methicillin-resistant Staphylococcus aureus isolated from raw milk of cows at small-scale production units. Journal of Advanced Veterinary and Animal Research, 9(1), 113–121. Retrieved from https://banglajol.info/index.php/JAVAR/article/view/79969

Issue

Vol. 9 No. 1 (2022)

Section

Original Articles

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Copyright (c) 2022 Hamza Mohamed Eid, Heba Sayed El-Mahallawy, Sahar Roshdi Mohammed, Nehal Ezzat Yousef Mohammed, Nada Hussein Eidaroos

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