Preliminary study on designing the binder of sperm-1 synthetic vaccine using sequence-based methods and molecular docking

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Keywords:

BSP1; epitope; peptide; ruminant; synthetic vaccine; T-cell

Abstract

Objective: The main objective of this study is to design a synthetic vaccine from the binder of sperm-1 (BSP1). Materials and Methods: This study was carried out using bioinformatics-related techniques. BSP-1 has been chosen as one of the biomarkers of a ruminant’s male fertility. We hypothesize that the BSP1 synthetic vaccines, which contain T-cell epitopes, can produce antibodies more effectively for the development of a sperm fertility detection kit. A sequence of BSP-1 peptides A0A0K1YXR5 from Bubalus bubalis (Domestic water buffalo) origin has been decided to be used to develop the peptide vaccine. Results: In this study, we succeeded in making synthetic vaccines from BSP-1 with a peptide sequence of LPEDSVPDEERVFPFTYRNRKHF. The three-dimensional theoretical prediction analysis of the peptide binding pattern to its ligand, as well as the molecular docking, has also been revealed. Conclusions: A synthetic vaccine from the BSP-1 has been developed in this study with the amino acid sequence LPEDSVPDEERVFPFTYRNRKHF, which is buffer-soluble, and the three-dimensional theoretical prediction analysis of the peptide binding pattern of BSP-1 to its ligand, as well as molecular docking, has also been revealed.

J. Adv. Vet. Anim. Res., 9(1): 122–127, March 2022

http://doi.org/10.5455/javar.2022.i576

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Published

2022-03-10

How to Cite

Wariata, W., Sriasih , M., Rosyidi , A., Ali , M., & Depamede, S. N. (2022). Preliminary study on designing the binder of sperm-1 synthetic vaccine using sequence-based methods and molecular docking. Journal of Advanced Veterinary and Animal Research, 9(1), 122–127. Retrieved from https://banglajol.info/index.php/JAVAR/article/view/79970

Issue

Vol. 9 No. 1 (2022)

Section

Original Articles

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Copyright (c) 2022 Wayan Wariata, Made Sriasih , Anwar Rosyidi , Muhamad Ali , Sulaiman Ngongu Depamede

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