Prevalence, antibiotic sensitivity profile, and phylogenetic analysis of Escherichia coli isolated from raw dromedary camel milk in Matrouh Governorate, Egypt

Authors

  • Elham Saeed Department of Food Hygiene, Faculty of Veterinary Medicine, Matrouh University, Mersa Matrouh, Egypt. https://orcid.org/0000-0001-6002-749X
  • Amr Abd El Moamen Amer Department of Food Hygiene, Faculty of Veterinary Medicine, Alexandria University, Alexandria, Egypt. https://orcid.org/0000-0002-5495-5828
  • Hani Gamal Keshta Department of Animal Medicine, Faculty of Veterinary Medicine, Matrouh University, Mersa Matrouh, Egypt.
  • Elsayed E Hafez Department of Plant Protection and Biomolecular Diagnosis, ALCRI, City of Scientific Research and Technological Applications Alexandria, Alexandria, Egypt. https://orcid.org/0000-0003-4044-5103
  • Rania M S Sultan Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
  • Eman Khalifa Department of Microbiology, Faculty of Veterinary Medicine, Matrouh University, Mersa Matrouh, Egypt. https://orcid.org/0000-0003-1029-9571

Keywords:

Escherichia coli; Matrouh Governorate; phoA gene; raw dromedary camel milk; sequencing

Abstract

Objective: Most people in Matrouh Governorate consume camel milk as a treatment for many diseases in a raw state to obtain nutritive value. Raw dromedary camel milk can be contaminated by Escherichia coli through fecal matter at any point of milk handling; therefore, it may lose its value and safety specifications. This survey aimed to estimate the incidence of E. coli in fresh camel milk. Materials and Methods: 100 fresh camel milk samples (50 from markets and 50 from farms) were randomly collected from different districts in Matrouh Governorate, Egypt, over 4 months for the detection of E. coli incidence through conventional bacterial isolation, molecular investigation, and gene sequencing. Results: The prevalence rates of E. coli in the examined market and farm raw camel milk based on conventional methods were 24% and 8%, respectively, while those by molecular identification using phoA as an E. coli determinate gene were 4% and 6%, respectively. Moreover, E. coli phoA gene phylogenetic analysis revealed high sequence similarity to E. coli strain CP033158.1 in India and E. coli strain CP047594.1 in China. Antibiotic sensitivity of E. coli isolates showed high susceptibility to norfloxacin (10 µg) and cefoperazone (75 µg). On the other hand, high resistance was found in rifamycin (30 µg) and cefoxitin (30 µg). Conclusion: The results indicate that market camel milk is more contaminated than the farms’ own. Additionally, antibiotic resistance is increasing due to antibiotic abuse.

J. Adv. Vet. Anim. Res., 9(1): 138–143, March 2022

http://doi.org/10.5455/javar.2022.i578

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Published

2022-03-13

How to Cite

Saeed, E., Amer, A. A. E. M., Keshta, H. G., Hafez, E. E., Sultan, R. M. S., & Khalifa, E. (2022). Prevalence, antibiotic sensitivity profile, and phylogenetic analysis of Escherichia coli isolated from raw dromedary camel milk in Matrouh Governorate, Egypt. Journal of Advanced Veterinary and Animal Research, 9(1), 138–143. Retrieved from https://banglajol.info/index.php/JAVAR/article/view/79975

Issue

Vol. 9 No. 1 (2022)

Section

Original Articles

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Copyright (c) 2022 Elham Saeed, Amr Abd El-Moamen Amer, Hani Gamal Keshta, Elsayed E Hafez, Rania M S Sultan, Eman Khalifa

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