A combination of taurine and caffeine maintains sperm quality in equine semen during chilled storage

Authors

  • Hermelinda Ramirez Perez Depto. de Reproducción, Facultad de Medicina Veterinaria Zootecnia, UNAM, Ciudad de México, México.
  • Hilda Morayma Guerrero Netro Depto. de Reproducción, Facultad de Medicina Veterinaria Zootecnia, UNAM, Ciudad de México, México.
  • Paulina Torres Rodríguez Instituto de Biotecnología, UNAM, Morelos, México.
  • Maricruz Díaz Durán Depto. de Reproducción, Facultad de Medicina Veterinaria Zootecnia, UNAM, Ciudad de México, México.
  • Ana Myriam Boeta Acosta Depto. de Reproducción, Facultad de Medicina Veterinaria Zootecnia, UNAM, Ciudad de México, México.
  • Mouhamadou Diaw Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Montreal, Saint-Hyacinthe, QC, Canada.

Keywords:

Equine; chilled semen; caffeine; taurine; motility; viability

Abstract

Objective: The objective of this study was to evaluate the effects of caffeine and taurine on the motility and viability of chilled equine semen.  Materials and Methods: A total of 12 ejaculates were collected from three mature stallions with proven fertility during the breeding season. The gel-free spermatic fraction of each ejaculate was divided into two aliquots and diluted with a semen extender (either INRA 96® or BotuSemen Gold®). The aliquots were then split and assigned to one of the six treatment groups: control (no supplement), caffeine (2 and 4 mM), taurine (25 and 50 mM), and a combination of caffeine (2 mM) plus taurine (25 mM). Samples were stored at 4°C and analyzed at different time points (0, 24, 48, 72, and 96 h) to evaluate total (TMOT) and progressive (PMOT) motility and viability by computer-assisted sperm analysis.  Results: Regardless of the extender, PMOT and TMOT decreased over time. However, compared with the control, the treatment with 4 mM caffeine significantly mitigated the decrease in PMOT at 72 h. Additionally, semen treated with a combination of caffeine plus taurine maintained a significantly higher PMOT at 96 h, with improved viability at all time points.  Conclusions: The combination of caffeine plus taurine helps maintain chilled equine semen viabil­ity and progressive motility up to 96 h independently of the extender used.

J. Adv. Vet. Anim. Res., 8(4): 635–641, December 2021

http://doi.org/10.5455/javar.2021.h555

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Published

2021-11-03

How to Cite

Perez, H. R., Netro, H. M. G., Rodríguez, P. T., Durán, M. D., Acosta, A. M. B., & Diaw, M. (2021). A combination of taurine and caffeine maintains sperm quality in equine semen during chilled storage. Journal of Advanced Veterinary and Animal Research, 8(4), 635–641. Retrieved from https://banglajol.info/index.php/JAVAR/article/view/80082

Issue

Vol. 8 No. 4 (2021)

Section

Original Articles

License

Copyright (c) 2025 Hermelinda Ramirez Perez, Hilda Morayma Guerrero Netro, Paulina Torres Rodríguez, Maricruz Díaz Durán, Ana Myriam Boeta Acosta, Mouhamadou Diaw

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