Genetic polymorphism of myostatin gene in Sumba Ongole (Bos indicus) cattle and its association with growth traits

Authors

  • Cynthia Dewi Gaina Laboratory of Veterinary Clinic, Reproduction, Pathology, and Nutrition, Faculty of Medicine and Veterinary Medicine, Universitas Nusa Cendana, Kupang, Indonesia. https://orcid.org/0000-0002-0322-1373
  • Filphin Adolfin Amalo Laboratory of Veterinary Anatomy, Physiology, Pharmacology and Biochemistry, Faculty of Medicine and Veterinary Medicine, Universitas Nusa Cendana, Kupang, Indonesia. https://orcid.org/0000-0003-0134-8773

Keywords:

Myostatin gene; polymorphism; Sumba Ongole cattle; Bos indicus; growth traits

Abstract

Objective: As one of the most valuable genetic resources of Ongole beef cattle globally, the Sumba Ongole (SO) cattle population is being studied in this investigation of myostatin (MSTN) gene polymorphism and its association with growth traits. Materials and Methods: Blood samples from 161 SO cattle were collected and analyzed. Deoxyribonucleic acid (DNA) was isolated. The DNA was electrophoresed and extracted, and finally, the annealing temperature was optimized, followed by amplification and sequencing. Next, we used a Basic local alignment search tool to assess the sequencing data. Results: The analysis revealed 22 single nucleotide polymorphisms (SNPs) in the MSTN gene in this region that showed genetic variation. Two SNPs, c.424 G > A, and c.467 G > C, were found to be significantly associated with SO cattle phenotypes of wither height, heart girth, and hip height (p < 0.05) but not with body weight or body length (p > 0.05). Conclusion: As a result of our findings, the MSTN gene polymorphism and its correlation with growth traits in SO cattle may be employed as a candidate marker in SO cattle and other beef cattle breeds.

J. Adv. Vet. Anim. Res., 9(4): 565–572, December 2022

http://doi.org/10.5455/javar.2022.i625

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Published

2022-11-18

How to Cite

Gaina , C. D., & Amalo, F. A. (2022). Genetic polymorphism of myostatin gene in Sumba Ongole (Bos indicus) cattle and its association with growth traits. Journal of Advanced Veterinary and Animal Research, 9(4), 565–572. Retrieved from https://banglajol.info/index.php/JAVAR/article/view/80174

Issue

Vol. 9 No. 4 (2022)

Section

Original Articles

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Copyright (c) 2022 Cynthia Dewi Gaina , Filphin Adolfin Amalo

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