Acute exacerbation of idiopathic pulmonary fibrosis model in the rats using bleomycin and lipopolysaccharides

Authors

  • Sandy Vitria Kurniawan Doctoral Program in Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Department of Pharmacology and Pharmacy, School of Medicine and Health Sciences, Atma Jaya Catholic University of Indonesia, Jakarta, Indonesia. https://orcid.org/0000-0002-1707-0166
  • Melva Louisa Department of Pharmacology and Therapeutics, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia. https://orcid.org/0000-0002-9451-0380
  • Jamal Zaini Department of Pulmonology and Respiratory Medicine Faculty of Medicine Universitas Indonesia, Persahabatan National Respiratory Referral Hospital, Jakarta, Indonesia. https://orcid.org/0000-0002-3573-6312
  • Silvia Surini Laboratory of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia. https://orcid.org/0000-0003-3345-1273
  • Vivian Soetikno Department of Pharmacology and Therapeutics, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.
  • Puspita Eka Wuyung Department of Anatomical Pathology, Faculty of Medicine Universitas Indonesia, Depok, Indonesia; Animal Research Facilities, Indonesian Medical Education and Research Institute, Faculty of Medicine Universitas Indonesia, Depok, Indonesia. https://orcid.org/0000-0003-3345-1273
  • Rosemary Ceria Tatap Uli Animal Research Facilities, Indonesian Medical Education and Research Institute, Faculty of Medicine Universitas Indonesia, Depok, Indonesia.

Keywords:

Acute exacerbation; bleomycin; bronchoalveolar lavage fluid; inflammation; idiopathic pulmonary fibrosis; lipopolysaccharides

Abstract

Objective: This study was conducted to establish a rat model of acute exacerbation of idiopathic pulmonary fibrosis (AE-IPF) using the combination of bleomycin (BLM) and lipopolysaccharides (LPS). Materials and Method: Twenty-four male Sprague Dawley rats were allocated into two equal groups: the sham or the bleomycin and lipopolysaccharides-induced AE-IPF group (BLM-LPS). On Day 7, BLM intratracheally and LPS intraperitoneally were both used to administer AE-IPF. The BLM-LPS group and its respective sham group were terminated on Days 8, 14, or 21. Samples of bronchoalveolar lavage fluid (BALF) and lungs were taken and investigated for cell count and histopathology. Results: On Day 8, histological analysis revealed inflammatory cell infiltration with edema and hyaline membrane, and the BALF differential cell count revealed high neutrophil counts. By having a higher collagen density area and Ashcroft modified score than the sham group on Day 14, the BLM-LPS group displayed significantly lower oxygen saturation, alveolar air area, and a fibrotic appearance. However, there was a spontaneous resolution in inflammation and fibrotic appearance on Day 21 after the BLM administration. Conclusions: By combining BLM and LPS, it was possible to create a successful rat model of AE-IPF. The present model showed the peak exacerbation on Day 8 and the fibrotic peak on Day 14, which gradually improved. The optimal time for the new AE-IPF therapeutic intervention was determined to be between Days 8 and 14.  

J. Adv. Vet. Anim. Res., 10(2): 196–204, June 2023

http://doi.org/10.5455/javar.2023.j669

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Published

2023-06-30

How to Cite

Kurniawan, S. V., Louisa, M., Zaini, J., Surini, S., Soetikno, V., Wuyung, P. E., & Uli, R. C. T. (2023). Acute exacerbation of idiopathic pulmonary fibrosis model in the rats using bleomycin and lipopolysaccharides. Journal of Advanced Veterinary and Animal Research, 10(2), 196–204. Retrieved from https://banglajol.info/index.php/JAVAR/article/view/80390

Issue

Vol. 10 No. 2 (2023)

Section

Original Articles

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Copyright (c) 2023 Sandy Vitria Kurniawan, Melva Louisa, Jamal Zaini, Silvia Surini, Vivian Soetikno, Puspita Eka Wuyung, Rosemary Ceria Tatap Uli

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