Detection of Aeromonas hydrophila possessing aerolysin gene using gold nanoparticle probe

Authors

  • Arren Christian M de Guia Livestock Biotechnology Center, Philippine Carabao Center, Science City of Muñoz, The Philippines ;Freshwater Aquaculture Center—College of Fisheries, Central Luzon State University, Science City of Muñoz, The Philippines. https://orcid.org/0000-0002-8846-5720
  • Mary Rose D Uy de Guia Production System and Nutrition Section, Philippine Carabao Center, Science City of Muñoz, The Philippines. https://orcid.org/0000-0002-9124-2042
  • Juvy J Monserate Department of Chemistry, College of Science, Central Luzon State University, Science City of Muñoz, The Philippines. https://orcid.org/0000-0002-0135-4949
  • Joel R Salazar Department of Chemistry, College of Science, Central Luzon State University, Science City of Muñoz, The Philippines. https://orcid.org/0000-0002-9390-7319
  • Ravelina R Velasco Freshwater Aquaculture Center—College of Fisheries, Central Luzon State University, Science City of Muñoz, The Philippines. https://orcid.org/0000-0003-4369-0366
  • Claro N Mingala Livestock Biotechnology Center, Philippine Carabao Center, Science City of Muñoz, The Philippines; Department of Animal Science, College of Agriculture, Institute of Graduate Studies, Central Luzon State University, Science City of Muñoz, The Philippines. https://orcid.org/0000-0002-0435-2156
  • Karl Marx A Quiazon Freshwater Aquaculture Center—College of Fisheries, Central Luzon State University, Science City of Muñoz, The Philippines. https://orcid.org/0000-0002-1633-0659

Keywords:

Aeromonas hydrophila; aerA gene; minimum detectable limit; PCR assay

Abstract

Objective: The aerolysin (aerA) is a virulence indicator used to identify the pathogenicity of the Aeromonas strain. Targeting a pathogen’s crucial virulence gene for detection is essential, as it determines the potential threat to the host. This study aimed to develop a gold nanoparticle (AuNP) probe for detecting the gene aerA in Aeromonas hydrophila among field samples. Materials and Methods: Kidney samples among both healthy and sick Nile tilapias in five provinces of Luzon Island were collected for bacterial analysis. Screening using specific primers targeting aerA was conducted in parallel with testing the AuNPs probe on the same sample set. The positive control provided by BFAR-NFLD, confirmed by polymerase chain reaction (PCR) assay, was used as a positive sample containing the target gene. Results: The AuNP probe demonstrated a computed accuracy of 81.32%, sensitivity of 100%, and specificity of 81.26%. Among the 257 reactions, 59 were false positives, while no false negative results were observed. The AuNP probe could detect aerA at levels as low as 30 ng/µl. The low prevalence of the target gene may be attributed to the use of general media instead of specific media like Rimler-Shotts agar. Conclusion: The established colorimetric detection method for A. hydrophila with the aerA gene offers a swift alternative to PCR, negating the requirement for advanced equipment like a thermal cycler.

J. Adv. Vet. Anim. Res., 10(4): 593–598, December 2023

http://doi.org/10.5455/javar.2023.j714

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Published

2023-12-31

How to Cite

Guia, A. C. M. de, Guia, M. R. D. U. de, Monserate, J. J., Salazar, J. R., Velasco, R. R., Mingala, C. N., & Quiazon, K. M. A. (2023). Detection of Aeromonas hydrophila possessing aerolysin gene using gold nanoparticle probe. Journal of Advanced Veterinary and Animal Research, 10(4), 593–598. Retrieved from https://banglajol.info/index.php/JAVAR/article/view/80728

Issue

Vol. 10 No. 4 (2023)

Section

Original Articles

License

Copyright (c) 2023 Arren Christian M de Guia, Mary Rose D Uy de Guia, Juvy J Monserate, Joel R Salazar, Ravelina R Velasco, Claro N Mingala, Karl Marx A Quiazon

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