EphB4 and ephrin-B1 expression in the intra-testicular-resident macrophages in mice.

Authors

  • Md Royhan Gofur Department of Veterinary and Animal Sciences, University of Rajshahi, Rajshahi, Bangladesh. https://orcid.org/0000-0002-8778-2417
  • Kazushige Ogawa Laboratory of Veterinary Anatomy, Graduate School of Veterinary Science, Osaka Metropolitan University, Izumisano, Osaka, Japan.

Keywords:

EphB4; ephrin-B1; F4/80; intratesticular-resident macrophages; tyrosine-phosphorylation

Abstract

Objective: The objective was to find out the expression of EphB4 receptor and ephrin-B1 ligand by the macrophages that live inside the mouse testicles. Materials and Methods: Messenger ribonucleic acid (mRNA) expression of EphB4 and ephrin-B1 was identified via RT-PCR amplification, and protein expression was examined by immunostaining. Results: Analysis using RT-PCR revealed that mRNA of EphB4 and ephrin-B1 were noticed in the examined testis of all postnatal ages. Furthermore, immunostaining revealed that F4/80-positive intra-testicular-resident macrophages were located in the intertubular spaces within the testis and more densely around the intra-testicular excurrent duct system, and increased in number gradually during the postnatal period of development until 5 weeks of age, when the mice attain their maturity (puberty), and maintained thereafter. Both EphB4 and ephrin-B1 immunoreactivity were noticed in F4/80-positive intra-testicular-resident macrophages within the testis of all studied postnatal ages. Ephrin-B1 and EphB4 immunoreactivity were weak during early postnatal development until the age of 2 weeks, and then ephrin-B1 immunoreactivity became very strong and EphB4 immunoreactivity became strong at the age of 3 weeks, and they continued to do so until the age of 8 weeks. Furthermore, EphB4 receptor was tyrosine-phosphorylated in testis. Conclusion: The expression of EphB4 and ephrin-B1 in mice intra-testicular-resident macrophages is being examined for the first time in this work. The localization of EphB4 and ephrin-B1, and EphB4 tyrosine-phosphorylation suggest that EphB4/ephrin-B1 signaling might occur in the intra-testicular-resident macrophages, and may participate in maintaining male fertility.

J. Adv. Vet. Anim. Res., 11(3): 746-753, September 2024

http://doi.org/10.5455/javar.2024.k826

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Published

2024-09-24

How to Cite

Gofur, M. R., & Ogawa, K. (2024). EphB4 and ephrin-B1 expression in the intra-testicular-resident macrophages in mice. Journal of Advanced Veterinary and Animal Research, 11(3), 746–753. Retrieved from https://banglajol.info/index.php/JAVAR/article/view/81089

Issue

Vol. 11 No. 3 (2024)

Section

Original Articles

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Copyright (c) 2024 Md Royhan Gofur, Kazushige Ogawa

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