Fertilization rate of crossbreeding cattle using sexing and conventional semen in different seasons in South Papua
Keywords:
Conception rate; semen; sexing; female cattle; THIAbstract
Objective: Fertilization rate of artificially inseminated cows using sexed and conventional semen in different seasons in South Papua. Materials and Methods: Eighty crossbred cows aged 4–4.5 years with body condition score 3.8 were divided into groups A (summer = 40 cows) and B (rainy season = 40 cows). Each cow in each season was artificial insemination (AI) using sexed frozen semen and conventional semen. Frozen semen was evaluated for post-thawing motility (PTM), cell membrane integrity, and acrosome damage before synchronization using 5 ml PGF2α plus vitamin E. Using a visual gun, we identified cows in estrus on days 4–7 post-synchronization. Pregnancy of cows was detected using N5Vet ultrasound on days 35 and 55. The interaction between season, semen type, and fertilization level was analyzed using standard error and two-way ANOVA, assisted by SPSS 21 software. Results: The wet season Temperature-Humidity Index (THI) level averaged 77.12 ± 1.19, and the summer season THI level averaged 82.67 ± 1.25. PTM quality averaged 60%–65%, viability 61%– 71%, sperm membrane integrity 62%–65%, and acrosome integrity 88%–91%. Conception rates (CR) value of rainy season (p < 0.05) with summer season. In addition, the services per conception (S/C) value in the rainy season (p > 0.05) is the same as in the summer. This study’s S/C and CR values were within normal limits, and the pregnancy rate reached 65%–86%. Pregnancy detection can be observed on day 35, and the fetal heartbeat is visible. Conclusion: Post-AI fertilization using conventional semen was better in all seasons. The double dose of sexed semen can increase the fertilization rate in summer.
J. Adv. Vet. Anim. Res., 11(4): 954–960, December 2024
Downloads
23
Published
How to Cite
Issue
Section
License
Copyright (c) 2024 Nurcholis Nurcholis, Lilik Sumaryanti, Apri Irianto, Syetiel Maya Salamony

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).