Use of ash filtrate as an alternative to chemical disinfectant and its antimicrobial efficacy

Authors

  • Khairun Nahar Shithi Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh; Bangladesh Livestock Research Institute, Savar, Bangladesh.
  • Ananna Saha Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh.
  • Md Nurul Haque Bangladesh Livestock Research Institute, Savar, Bangladesh.
  • Ismail Hossain Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh. https://orcid.org/0009-0007-1543-9445
  • Mohammed Nooruzzaman Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh. https://orcid.org/0000-0002-9358-1494
  • Jahan Ara Begum Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh. https://orcid.org/0000-0002-3392-2496
  • Rokshana Parvin Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh.
  • Emdadul Haque Chowdhury Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh. https://orcid.org/0000-0002-8212-7923

Keywords:

Household ash; hand-made filtration system; ash filtrate (AF); antimicrobial efficacy; disinfectant;sanitizer

Abstract

Objective: The study aims to prepare the ash filtrate (AF) from household ashes using an in-house-designed handmade filtration system and to observe the antimicrobial efficacy and sanitizing effects. Materials and Methods: Household ashes from various plant sources were collected, and AF was prepared through a handmade filtration system after adding water. The pH of stock AF was measured, and 20%, 40%, 60%, and 80% AF solutions were prepared by adding distilled water in appropriate proportions to obtain a wide range of pH values. The antimicrobial efficacy of AF against Salmonella spp. in vitro, Newcastle disease virus (NDV), and low pathogenic avian influenza virus (LPAIV) H9 N2 in ovo were analyzed. Contaminated eggs were individually sprayed or dipped with AF to detect the bacterial load on the eggshell surface. Further experimental use of AF as an egg sanitizer in routine biosecurity operations in broiler sheds was also evaluated. Results: The prepared AF showed high alkalinity; pH varied from 10.7 to 8.20 and contained a higher amount of K, Na, and Cl. The alkaline AF and its dilution gradually inhibited Salmonella growth and showed gradual pH-dependent antibacterial efficacy. Similarly, AF and its dilution showed a gradual decrease in viral titer against the LPAIV (H9N2); however, antiviral activity against the velogenic strain of NDV was quite steady. Applying AF as an egg sanitizer also reduced the bacterial loads significantly on the eggshell surface compared to untreated eggs. Moreover, AF having pH 10.5 experimentally used in routine sanitization practices of a boiler shed resulted in low bird mortality (10/210), higher body weight gain, and a low feed conversion ratio compared to the untreated control flock. Conclusion: The higher alkalinity of the AF is responsible for the antimicrobial activity of commercial disinfectants. Consequently, we can use AF as a low-cost, effective, natural antimicrobial agent to replace chemical disinfectants.

J. Adv. Vet. Anim. Res., 11(4): 1007–1016, December 2024

http://doi.org/10.5455/javar.2024.k851

Downloads

Download data is not yet available.
Abstract
20
PDF
40

Downloads

Published

2024-12-28

How to Cite

Shithi, K. N., Saha, A., Haque, M. N., Hossain, I., Nooruzzaman, M., Begum, J. A., … Chowdhury, E. H. (2024). Use of ash filtrate as an alternative to chemical disinfectant and its antimicrobial efficacy. Journal of Advanced Veterinary and Animal Research, 11(4), 1007–1016. Retrieved from https://banglajol.info/index.php/JAVAR/article/view/81184

Issue

Vol. 11 No. 4 (2024)

Section

Original Articles

License

Copyright (c) 2024 Khairun Nahar Shithi, Ananna Saha, Md Nurul Haque, Ismail Hossain, Mohammed Nooruzzaman, Jahan Ara Begum, Rokshana Parvin, Emdadul Haque Chowdhury

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Authors who publish with this journal agree to the following terms:

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).