Effect of narrowband ultraviolet B (311 nm) exposure on skin carcinogenesis in Wistar rats

Authors

  • Roro Inge Ade Krisanti Doctoral Program in Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Department of Dermatology and Venereology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia. https://orcid.org/0009-0005-4015-2046
  • Septelia Inawati Wanandi Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Center of Hypoxia and Oxidative Stress Studies, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia ; Molecular Biology and Proteomic Core Facilities, Indonesian Medical Education and Research Institute, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia. https://orcid.org/0000-0002-7963-8853
  • Puspita Eka Wuyung Doctoral Program in Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Department of Pathological Anatomy, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia. https://orcid.org/0000-0002-9737-4409
  • Aida S D Hoemardani Doctoral Program in Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Department of Dermatology and Venereology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia. https://orcid.org/0009-0004-0310-1859

Keywords:

Narrowband UVB; Skin carcinogenesis; Wistar rat

Abstract

Objective: The aim of this study is to determine narrowband UVB (NB-UVB) irradiation’s effect on the promotion of skin cancer, particularly its effect on DNA damage, oxidative stress, inflammation, and histological changes in Wistar rat skin. Materials and Methods: Wistar rats were selected for this study and randomly divided into control, dimethylbenzanthracene (DMBA), and DMBA+NB-UVB groups. The rats were given a single dose of DMBA and exposed to NB-UVB 3 times a week for 10 weeks. The radiation dose started with 1 minimal erythema dose, which is equivalent to 3.192 J/cm². In the 11th week, analysis on cyclobutene pyrimidine dimer (CPD), malondialdehyde (MDA), nuclear factor kappa-B (NFκB), inflammatory cytokines, and histopathology examination of the skin tissue was conducted. Results: Higher CPD, MDA, NFκB, tumor necrosis factor a (TNF-a), interleukin (IL)-6, IL-11, IL-10, and IL-12 levels in rats exposed to DMBA+NB-UVB for 10 weeks compared to control and DMBA groups. Macroscopic examination presented erythema, skin thickening, desquamation, ulcer, and crust. Histopathology examination showed hyperkeratosis, acanthosis, atypical keratinocytes, irregular arrangement of the basement membrane, and inflammatory cell infiltration in the DMBA+NB-UVB group. Conclusion: This research has shown that 10 weeks of a combination of DMBA and NB-UVB irradiation induced DNA damage, oxidative stress, inflammation, and histological changes in the Wistar rat skin.

J. Adv. Vet. Anim. Res., 11(4): 1105–1113, December 2024

http://doi.org/10.5455/javar.2024.k861

Downloads

Download data is not yet available.
Abstract
31
PDF
21

Downloads

Published

2024-12-29

How to Cite

Krisanti, R. I. A., Wanandi, S. I., Wuyung, P. E., & Hoemardani, A. S. D. (2024). Effect of narrowband ultraviolet B (311 nm) exposure on skin carcinogenesis in Wistar rats. Journal of Advanced Veterinary and Animal Research, 11(4), 1105–1113. Retrieved from https://banglajol.info/index.php/JAVAR/article/view/81208

Issue

Vol. 11 No. 4 (2024)

Section

Original Articles

License

Copyright (c) 2024 Roro Inge Ade Krisanti, Septelia Inawati Wanandi, Puspita Eka Wuyung, Aida S D Hoemardani

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Authors who publish with this journal agree to the following terms:

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).