Isolation of antibiotic-resistant strains of Staphylococcus aureus from raw milk produced by dairy cows with subclinical bovine mastitis.

Authors

  • Saima Batool Department of Wildlife and Ecology, University of Veterinary and Animal Sciences (UVAS), Lahore, Pakistan.
  • Zubia Masood Department of Zoology, Sardar Bahadur Khan Women’s University, Quetta, Pakistan. https://orcid.org/0000-0001-5083-1644
  • Asim Ullah Department of Fisheries and Aquaculture, University of Veterinary and Animal Sciences (UVAS), Lahore, Pakistan.
  • Wali Khan Department of Zoology, University of Malakand, Chakdara Dir Lower, Khyber Pakhtunkhwa, Pakistan. https://orcid.org/0000-0002-9273-3257
  • Mourad Ben Said Laboratory of Microbiology, National School of Veterinary Medicine of Sidi Thabet, University of Manouba, Sidi Thabet, Tunisia; Department of Basic Sciences, Higher Institute of Biotechnology of Sidi Thabet, University of Manouba, Sidi Thabet, Tunisia. https://orcid.org/0000-0001-5567-4721
  • Hanène Belkahia Laboratory of Microbiology, National School of Veterinary Medicine of Sidi Thabet, University of Manouba, Sidi Thabet, Tunisia.
  • Alaa Bassuny Ismael Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Taif University, Taif, Saudi Arabia.
  • Ayman A Swelum Department of Theriogenology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt. https://orcid.org/0000-0003-3247-5898

Keywords:

Staphylococcus aureus; antibiotic resistance; cow milk; dairy farms; Lahore

Abstract

Objectives: The rise of antibiotic-resistant strains of Staphylococcus aureus in dairy milk products is a global concern, compromising treatment efficacy and highlighting the need for innovative solutions. Therefore, a study was conducted to isolate S. aureus strains (N = 21) from raw milk samples of cows infected with subclinical bovine mastitis. Additionally, the resistance of these strains against 12 different antibiotics was examined. Materials and Methods: Sixty raw cow milk samples, 20 from each of three separate dairy farms in Lahore city, were collected and screened for the presence of S. aureus. It was discovered that 70% of these milk samples were contaminated with this bacterium, indicating a widespread presence across the farms. Different isolation tests were employed in this study, including gram staining, capsule staining, catalase, mannitol salt fermentation, DNase, coagulase, and oxidase. Results: The obtained results revealed that the isolated strains of S. aureus showed % of their resistance against different antibiotics in the order of amoxicillin (85%), penicillin (71%), gentamicin (CN) (42%), carbenicillin and trimethoprim-sulfamethoxazole (33%), streptomycin, ciprofloxacin, and oxytetracycline (28%), cefotaxime (10%), and chloramphenicol (4%) in decreasing order, respectively. However, these strains showed no resistance against Bacitracin and Ampicillin. Conclusion: The existence of resistant strains of S. aureus has been attributed to various factors, such as poor milk hygiene, delayed milk transportation, subclinical bovine mastitis among dairy cows, and antibiotic-resistant genes. Thus, our present study will provide useful information about the resistant strains of S. aureus, which may transfer through cows into milk and then produce serious food-borne diseases in human beings. This study will be helpful to improve and control the quality of dairy products in Pakistan.

J. Adv. Vet. Anim. Res., 12(1): 252–259, March 2025

http://doi.org/10.5455/javar.2025.l892

Downloads

Download data is not yet available.

Downloads

Published

2025-03-24

How to Cite

Batool, S., Masood, Z., Ullah, A., Khan, W., Said, M. B., Belkahia, H., … Swelum, A. A. (2025). Isolation of antibiotic-resistant strains of Staphylococcus aureus from raw milk produced by dairy cows with subclinical bovine mastitis. Journal of Advanced Veterinary and Animal Research, 12(1), 252–259. Retrieved from https://banglajol.info/index.php/JAVAR/article/view/82304

Issue

Vol. 12 No. 1 (2025)

Section

Original Articles

License

Copyright (c) 2025 Saima Batool, Zubia Masood, Asim Ullah, Wali Khan, Mourad Ben Said, Hanène Belkahia, Alaa Bassuny Ismael, Ayman A Swelum

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Authors who publish with this journal agree to the following terms:

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).