Molecular identification of antibiotic-resistant and virulent Escherichia coli and Staphylococcus aureus isolated from dogs in Southern Benin.

Authors

  • Ayaovi Bruno Yaovi Research Unit on Communicable Diseases, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Abomey-Calavi, Benin. https://orcid.org/0000-0003-2007-5643
  • Arpita Das Department of Biosciences and Bioengineering, Indian Institute of Technology Roorkee, Roorkee, India.
  • Rama N Behera Department of Biosciences and Bioengineering, Indian Institute of Technology Roorkee, Roorkee, India.
  • Prudencio Comlan Sossa-Minou Research Unit on Communicable Diseases, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Abomey-Calavi, Benin.
  • Vinod Singh Bisht Department of Biosciences and Bioengineering, Indian Institute of Technology Roorkee, Roorkee, India.
  • Monica Yadav Department of Biosciences and Bioengineering, Indian Institute of Technology Roorkee, Roorkee, India.
  • Ayushi Kapoor Department of Biosciences and Bioengineering, Indian Institute of Technology Roorkee, Roorkee, India.
  • François Dossa Research Unit on Communicable Diseases, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Abomey-Calavi, Benin.
  • Paulin Azokpota Laboratory of Food Science and Technology, University of Abomey-Calavi, Cotonou, Benin. https://orcid.org/0000-0001-7570-0042
  • Souaïbou Farougou Research Unit on Communicable Diseases, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Abomey-Calavi, Benin. https://orcid.org/0000-0003-3404-9542
  • Lamine Baba-Moussa Laboratory of Biochemistry and Molecular Typing in Microbiology, Department of Biochemistry and Cell Biology, Faculty of Science and Technology, University of Abomey-Calavi, Abomey-Calavi, Benin. https://orcid.org/0000-0001-7570-0042
  • Kiran Ambatipudi Department of Biosciences and Bioengineering, Indian Institute of Technology Roorkee, Roorkee, India. https://orcid.org/0000-0003-4157-0678
  • Philippe Sessou Research Unit on Communicable Diseases, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Abomey-Calavi, Benin. https://orcid.org/0000-0003-4154-9211

Keywords:

Prevalence; Escherichia coli; Staphylococcus aureus; Dogs; Benin.

Abstract

Objective: Antibiotic resistance is a worldwide health challenge. Close interaction with dogs carrying antibiotic-resistant zoonotic agents poses a risk to human health. The present study aimed to characterize antibiotic-resistant Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) isolated from dogs in Southern Benin. Materials and methods: A total of 336 swabs (112 buccal, 112 nasal, and 112 rectal) from 112 dogs living in the communes of Abomey-Calavi and Cotonou were analyzed for E. coli and S. aureus presence. Bacterial isolates were tested for antibiotic (penicillins, tetracyclines, aminoglycosides, cephalosporins, sulfonamides, and macrolides) susceptibility using the disc diffusion method, and antibiotic-resistant strains were characterized by the polymerase chain reaction method. Results: A 41.07% and 20.53% of dogs harbored E. coli and S. aureus, respectively. Escherichia coli and S. aureus isolates showed resistance to penicillin (100% and 81.48%), tetracycline (44.64% and 59.26%), and other antimicrobials tested. Escherichia coli isolates harbored resistance genes blaTEM (63.46%), tetA (62.50%), and strA-strB (55.56%). tetK (100%), tetM (100%), and blaZ (82.61%) were present in S. aureus isolates. Escherichia coli strains harbored virulence genes fimH (61.54%), kpsMTII (26.92%), fyuA (19.23%), and eae (1.92%), whereas 20.83% of S. aureus strains harbored pvl and fnbA. Conclusion: The results of the current study reveal the urgent need for stricter controls on antibiotic use. Implementing guidelines, responsible prescribing, and increasing public awareness are crucial steps to address this problem.

J. Adv. Vet. Anim. Res., 12(2): 396–419, June 2025

http://doi.org/10.5455/javar.2025.l907

Downloads

Download data is not yet available.
Abstract
8
PDF
8

Downloads

Published

2025-09-17

How to Cite

Yaovi, A. B., Das, A., Behera, R. N., Sossa-Minou, P. C., Bisht, V. S., Yadav, M., … Sessou, P. (2025). Molecular identification of antibiotic-resistant and virulent Escherichia coli and Staphylococcus aureus isolated from dogs in Southern Benin. Journal of Advanced Veterinary and Animal Research, 12(2), 396–419. Retrieved from https://banglajol.info/index.php/JAVAR/article/view/83533

Issue

Vol. 12 No. 2 (2025)

Section

Original Articles

License

Copyright (c) 2025 Ayaovi Bruno Yaovi, Arpita Das, Rama N Behera, Prudencio Comlan Sossa-Minou, Vinod Singh Bisht, Monica Yadav, Ayushi Kapoor, François Dossa, Paulin Azokpota, Souaïbou Farougou, Lamine Baba-Moussa, Kiran Ambatipudi, Philippe Sessou

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Authors who publish with this journal agree to the following terms:

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).