Effect of interferon on broilers’ fecal microbiome composition.

Authors

  • Inna Burakova Laboratory of Metagenomics and Food Biotechnology, Voronezh State University of Engineering Technologies, Voronezh, Russia. https://orcid.org/0000-0002-5881-0845
  • Yuliya Smirnova Laboratory of Metagenomics and Food Biotechnology, Voronezh State University of Engineering Technologies, Voronezh, Russia. https://orcid.org/0000-0002-5820-1804
  • Mariya Gryaznova Laboratory of Metagenomics and Food Biotechnology, Voronezh State University of Engineering Technologies, Voronezh, Russia. https://orcid.org/0000-0003-2076-3868
  • Polina Morozova Laboratory of Metagenomics and Food Biotechnology, Voronezh State University of Engineering Technologies, Voronezh, Russia. https://orcid.org/0009-0000-0075-9170
  • Vyacheslav Kotarev All-Russian Veterinary Research Institute of Pathology, Pharmacology and Therapy, Voronezh, Russia.
  • Ludmila Lyadova All-Russian Veterinary Research Institute of Pathology, Pharmacology and Therapy, Voronezh, Russia.
  • Nadezhda Ivanova All-Russian Veterinary Research Institute of Pathology, Pharmacology and Therapy, Voronezh, Russia.
  • Larisa Denisenko All-Russian Veterinary Research Institute of Pathology, Pharmacology and Therapy, Voronezh, Russia.
  • Mikhail Syromyatnikov Laboratory of Metagenomics and Food Biotechnology, Voronezh State University of Engineering Technologies, Voronezh, Russia; Department of Genetics, Cytology and Bioengineering, Voronezh State University, Voronezh, Russia. https://orcid.org/0000-0001-9028-0613

Keywords:

Interferon; fecal microbiome; broiler; sequencing

Abstract

Objective: The purpose of our study was to investigate the effect of chicken interferon on the intestinal microbiota of broiler chickens. Materials and Methods: The study used next-generation sequencing on the Ion Torrent pragmatic general multicast platform to target the V3 16S ribosomal ribonucleic acid hypervariable region gene, allowing us to analyze in detail changes in the composition of the broiler chicken microbiome. Results: Forty-one bacterial genera were identified in the studied groups of broilers. The highest abundance in both groups was observed for Lactobacillus, which was 31.08% ± 6.52 in the control group and 36.08% ± 7.25 in the interferon group. There was no clustering between the microbiome communities of the groups studied. We found a decrease or complete absence of Escherichia–Shigella, Eubacterium fissicatena group, Lachnospiraceae CHKCI001, and Pediococcus in the interferon-treated broiler group compared to the control group and an increase in the number of genera Ruminococcaceae CAG-352 and Turicibacter in the interferon group. Conclusion: A decrease in E.–Shigella may indicate normalization of the intestinal microbiota of broilers, and it can also be concluded that the introduction of interferon helps to suppress opportunistic bacteria. In the interferon group, a sharp increase in the number of Turicibacter was observed. Representatives of this genus are among the most common members in the intestines of broilers.

J. Adv. Vet. Anim. Res., 12(2): 487–496, June 2025

http://doi.org/10.5455/javar.2025.l914

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Published

2025-09-17

How to Cite

Burakova, I., Smirnova, Y., Gryaznova, M., Morozova, P., Kotarev, V., Lyadova, L., … Syromyatnikov, M. (2025). Effect of interferon on broilers’ fecal microbiome composition. Journal of Advanced Veterinary and Animal Research, 12(2), 487–496. Retrieved from https://banglajol.info/index.php/JAVAR/article/view/83561

Issue

Vol. 12 No. 2 (2025)

Section

Original Articles

License

Copyright (c) 2025 Inna Burakova, Yuliya Smirnova, Mariya Gryaznova, Polina Morozova, Vyacheslav Kotarev, Ludmila Lyadova, Nadezhda Ivanova, Larisa Denisenko, Mikhail Syromyatnikov

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