Establishment of Vero cell-adapted thermostable candidate Newcastle Disease vaccine strain

Authors

  • Md Humayun Kabir Laboratory of Animal Health, Cooperative Division of Veterinary Sciences, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Fuchu-shi, Japan; Bangladesh Livestock Research Institute, Savar, Bangladesh. https://orcid.org/0000-0002-5468-3877
  • Md Amirul Hasan Laboratory of Animal Health, Cooperative Division of Veterinary Sciences, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Fuchu-shi, Japan; Bangladesh Livestock Research Institute, Savar, Bangladesh. https://orcid.org/0000-0001-5430-2387
  • Yu Miyaoka Laboratory of Animal Health, Cooperative Division of Veterinary Sciences, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Fuchu-shi, Japan.
  • Makiko Yamaguchi Laboratory of Animal Health, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu-shi, Japan.
  • Chisaki Kadota Laboratory of Animal Health, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu-shi, Japan.
  • Dany Shoham Begin-Sadat Center for Strategic Studies, Bar-Ilan University, Ramat Gan, Israel.
  • Harumi Murakami Laboratory of Animal Health, Cooperative Division of Veterinary Sciences, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Fuchu-shi, Japan; Laboratory of Animal Health, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu-shi, Japan.
  • Kazuaki Takehara Laboratory of Animal Health, Cooperative Division of Veterinary Sciences, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Fuchu-shi, Japan; Laboratory of Animal Health, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu-shi, Japan.

Keywords:

Antibody; Newcastle disease virus; thermostability; vaccine; Vero cell.

Abstract

Objective: The current study aims to determine a thermostable Avian Orthoavulavirus 1 (AOaV1) or Newcastle disease (ND) vaccine candidate strain isolated from a duck and its adaptation to Vero cells. Material and Methods: From field isolates of AOaV-1 in Japan, avirulent APMV/northern pintail/ Japan/Aomori/2003 (dk-Amori/03, AOaV-1-261) was selected for its excellent thermostability, and the strain was heat treated at 56°C for 30 min during each passage into Vero cells to maintain thermostability and to adapt to Vero cells up to 20 passages, then named AOaV-1-Vero20. Growth curves were tested in Vero cells in comparison to the original AOaV-1-261. The intracerebral pathogenicity index (ICPI) test was done. The thermostability of the virus was checked at different temperatures. Serum neutralization test was done to check the induced levels of antibodies of the candidate vaccine strain in chicks. Sequencing of the HN and F genes and complete genome sequencing of the strains were done. Results: AOaV-1-Vero20 grew well on Vero cells compared to the original AOaV-1-261. The ICPI test score was 0. Minimum titer drops were found in the thermostability test. Induced, adequate levels of antibodies were measured using the serum neutralization test. Sequencing of the HN gene also revealed transmembrane domains, including 315 and 369 amino acid residues, with the same amino acids as those found in other thermostable strains. The sequence analysis results showed no significant variance in the whole genome sequences of AOaV-1-Vero20 and the original AOaV-1-261. Conclusion: In this study, AOaV-1-Vero20 was identified as a candidate vaccine strain that is thermostable, Vero cell-adapted, and has immunogenic potential.

J. Adv. Vet. Anim. Res., 12(3): 1001–1011, September 2025

http://doi.org/10.5455/javar.2025.l959

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Published

2025-12-29

How to Cite

Kabir, M. H., Hasan, M. A., Miyaoka, Y., Yamaguchi, M., Kadota, C., Shoham, D., … Takehara, K. (2025). Establishment of Vero cell-adapted thermostable candidate Newcastle Disease vaccine strain. Journal of Advanced Veterinary and Animal Research, 12(3), 1001–1011. Retrieved from https://banglajol.info/index.php/JAVAR/article/view/86656

Issue

Vol. 12 No. 3 (2025)

Section

Original Articles

License

Copyright (c) 2025 Md Humayun Kabir, Md Amirul Hasan, Yu Miyaoka, Makiko Yamaguchi, Chisaki Kadota, Dany Shoham, Harumi Murakami, Kazuaki Takehara

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