Callus Induction and Plantlet Regeneration from <i>In Vivo</i> Nodal and Internodal Segments and Shoot Tip of <i>Dalbergia sissoo</i> Roxb.

Abstract

A complete and efficient protocol was developed for in vitro callus induction, somatic embryogenesis and plantlet regeneration from in vivo nodal and internodal segments and shoot tips of sissoo (Dalbergia sissoo Roxb.). The highest percentage of callus induction (100%) was achieved from nodal segment on MS medium supplemented with 2.0 mg/1 BAP + 0.5 mg/1 NAA. This combination also produced highest fresh weight (5.4 g.) of callus per culture and produced friable green callus. The highest number of shoots (2.4) per explant was recorded on MS medium supplemented with 1.5 mg/1 BAP + 0.5 mg/1 IAA for internode segment. The maximum number of roots (5.1)per shoot was observed in the MS medium having 1.0 mg/1 IBA + 2.0 mg/1 NAA. The well Rooted plantlets were acclimatized and successfully established into soil in the natural condition where 60% plantlets were survived.

Keywords: Dalbergia sissoo, nodal segment, internodal segments, shoot tip, callus, embryo, regeneration.  

DOI:10.3329/jbs.v16i0.3740

J. bio-sci. 16: 41-48, 2008