<i>In Vitro</i> Indirect Plantlet Regeneration from Hypocotyl Segments and Cotyledonary Explant Derived Calli in Lady's Finger (<i>Abelmoschus esculentus</i> L. Monech)

Abstract

An experiment was conducted to develop an efficient protocol for in vitro regeneration of plantlets from hypocotyl segments and cotyledonary explants of Abelmoschus esculentus L. Monech. In this investigation the cultivar OK-285 of Abelmoschus esculentus L. Monech was used. At first somatic embryogenic calli were induced using MS medium supplemented with different concentrations of auxin and cytokinin singly or in combination. The cotyledonary explants showed the best callus induction rate (87.9%) in MS medium containing 1.5mgl^-l 2.4-D + 0.1mgl^-l BAP hormonal concentration while hypocotyl segments showed 82.6% callus induction rate in the same medium. For shoot formation, calli were subcultured on MS solid medium and the hypocotyl segments showed the best result (72.1%) in MS medium containing 2.0 mgl^-l BAP + 0.1 mgl^-l IAA and the mean number of shoots per callus was recorded 4.2. For root induction from shoots MS and ½MS media were used. The highest 63.5% microshoots initiated roots in ½ MS + 0.1 mgl^-l IBA medium and the highest mean number of root was 4.8. Rooted shoots were acclimated and successfully established into soil under natural condition with 70% survival.

Key words: Abelmoschus esculentus, hypocotyl segments and cotyledon, embryogenic calli, regeneration.  

DOI:10.3329/jbs.v16i0.3741

J. bio-sci. 16: 49-57, 2008