Detection of Imipenem Resistant Extended Spectrum B-Lactamases (Esbls) Producers Among Gram-Negative Bacilli in Chittagong Medical College Hospital, Chittagong

Abstract

Background: Extended-Spectrum b-lactamases (ESBLs) producing bacteria are increasing in number and causing more severe infections because of their continuous mutation and multidrug resistance property which make its treatment difficult. The emergence of carbapenem (Imipenem) resistantant ESBLs will worsen the management of infections and increases the mortality rates. The present study was undertaken to detect the imipenem resistantant ESBLs producing bacteria in patients attending Chittagong Medical College Hospital. Materials and methods: All the isolates from different clinical samples were identified by standard procedure of identification & antibiotic sensitivity pattern were done accordingly. Isolated gramnegative bacteria were initially screened by Minimum Inhibitory Concentration (MIC) ESBLs breakpoints & then confirmed by Phenotypic Confirmatory Test (PCT). Results: In the present study, 94.67% were found as suspected ESBLs producers, of which 62.68% confirmed as ESBL producers. The prevalence of ESBLs producers was found to be 59.33%, where Klebsiella species (67.50%) was the leading ESBLs producers. Among them 6.74% were imipenem resistant ESBLs producers. Conclusion: It is essential to report ESBL production along with routine antimicrobial sensitivity testing time to time for the selection of antibiotics for empirical treatment.

JCMCTA 2017 ; 28 (2) : 69-74