Glucose induced intracellular ionic change in pancreatic islets of rat on inhibition of Na+/K+ pump by ouabain and its relation with insulin secretion

Authors

  • AN Chowdhury Professor (c.c.), Department of Biochemistry, Dhaka National Medical College
  • S Saha Associate Professor, Department of Pharmacology, Dhaka National Medical College
  • S Saha Lecturer, Department of Biochemistry, Dhaka National Medical College
  • L Ali Vice-chancellor, Bangladesh University of Health Science, Darus-Salam Road, Mirpur, Dhaka

DOI:

https://doi.org/10.3329/jdnmch.v19i2.77740

Keywords:

Na+/K+ pump, Ouabain, Pancreatic islets

Abstract

Glucose is the primary physiological stimulator of insulin release. Na+/K+ pump has a major role in modulating the glucose action. Rat pancreatic islets were isolated by collagenase digestion. The concentration of ions in the homogenized islets were measured by Ion-sensitive Electrode based autoanalyzer. Inhibition of Na+/K+ pump by ouabain resulted in gradual increase of intracellular Na+, decrease of K+, increase of Ca++ and decrease of Mg++ compared to the physiological medium. The changes of K+ paralleled by a rise of Na+ in the media indicate that a pump-related effect seems to be more plausible for the difference in ionic changes.

J.Dhaka National Med. Coll. Hos. 2013; 19 (02): 46-48

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Published

2013-09-30

How to Cite

Chowdhury, A., Saha, S., Saha, S., & Ali, L. (2013). Glucose induced intracellular ionic change in pancreatic islets of rat on inhibition of Na+/K+ pump by ouabain and its relation with insulin secretion. Journal of Dhaka National Medical College &Amp; Hospital, 19(2), 46–48. https://doi.org/10.3329/jdnmch.v19i2.77740

Issue

Vol. 19 No. 2 (2013)

Section

Original Articles