Evaluation of Hepatoprotective and In-vitro Cytotoxic Activity of Leaves of Premna serratifolia Linn

Authors

  • Rajendran Vadivu lecturer
  • A. Jerad Suresh Madras Medical College
  • K Girinath Madras Medical College India
  • P. Boopathi Kannan Madras Medical College, India
  • R Vimala Madras Medical College, India
  • N. M. Satish Kumar Madras Medical College, India

DOI:

https://doi.org/10.3329/jsr.v1i1.1046

Keywords:

Premna serratifolia Linn, Alcoholic extract hepatoprotective, In-vitro cytotoxic activity.

Abstract

Premna serratifolia is used by the traditional practitioners as cardiotonic, antibiotic, anti-coagulant, stomachic, carminative, hepatoprotective, antitumor etc. The present study aims in the evaluation of hepatoprotective and in-vitro cytotoxic activity of alcoholic extract of leaves of Premna serratifolia Linn. Hepatoprotective activity is studied by carbon tetrachloride induced hepato-toxicity in rats and the in-vitro cytotoxic activity is carried out by tryphane blue exclusion method using EAC cell lines. The degree of protection in hepatoprotective activity has been measured by using biochemical parameters such as serum glutamate oxalate transaminase (SGOT) and serum glutamate pyruvate transaminase (SGPT), alkaline phosphatase (ALP), bilirubin and total protein. The results suggest that the alcoholic extract at the dose level of 250mg/kg has produced significant  (p < 0.001) hepatoprotection by decreasing the activity of serum enzymes, bilirubin, and lipid peroxidation which is comparable to that of standard drug silymarin. The alcoholic extract also does exhibit the IC50 value of 75µg/ml which indicates the significant in-vitro cytotoxic activity of the extract. It is concluded that alcoholic extract of leaves of Premna serratifolia Linn is not only an effective hepatoprotective agent, but also possesses significant antitumor activity.

 

Keywords: Premna serratifolia Linn; Alcoholic extract hepatoprotective; In-vitro cytotoxic activity.

 

© 2009 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights

reserved.

 

DOI: 10.3329/jsr.v1i1.1046

 

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Author Biographies

Rajendran Vadivu, lecturer

Lecturer
Department of Pharmacognosy, College of Pharmacy, Madras Medical College, Chennai-03, India

A. Jerad Suresh, Madras Medical College

Department of Pharmacognosy, College of Pharmacy, Madras Medical College, Chennai-03, India

K Girinath, Madras Medical College India

Department of Pharmacognosy, College of Pharmacy, Madras Medical College, Chennai-03, India

P. Boopathi Kannan, Madras Medical College, India

Department of Pharmacognosy, College of Pharmacy, Madras Medical College, Chennai-03, India

R Vimala, Madras Medical College, India

Department of Pharmacognosy, College of Pharmacy, Madras Medical College, Chennai-03, India

N. M. Satish Kumar, Madras Medical College, India

Department of Pharmacognosy, College of Pharmacy, Madras Medical College, Chennai-03, India

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Published

2008-12-24

How to Cite

Vadivu, R., Suresh, A. J., Girinath, K., Kannan, P. B., Vimala, R., & Kumar, N. M. S. (2008). Evaluation of Hepatoprotective and In-vitro Cytotoxic Activity of Leaves of Premna serratifolia Linn. Journal of Scientific Research, 1(1), 145–152. https://doi.org/10.3329/jsr.v1i1.1046

Issue

Section

Section B: Chemical and Biological Sciences