A HPTLC Densitometer Determination of Sinapic acid in Chandrasur (Lepidium sativum)
Keywords:Lepidium sativum, Sinapic acid, HPTLC, Photo densitometry
A qualitative and quantitative analysis method was established to improve quality assessment standards for Lepidium sativum seeds. The sinapic acid was identified by comparing with standards and quantified simultaneously by High performance thin layer chromatographic (HPTLC). HPTLC of Lepidium sativum methanolic extract was performed on Silica gel 60F254 [20 cm Ã 10 cm] plates with butanol: acetic acid: water (4:1:5), as mobile phase. Quantitative evaluation of the plate was performed in the absorbance-reflectance mode at 326 nm. The sinapic acid was separated on a thin layer of silica gel and determined by HPTLC-photo densitometry. The proposed method is simple, sensitive and can be used for the routine assay.
Keywords: Lepidium sativum; Sinapic acid; HPTLC; Photo densitometry.
Â© 2009 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights
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