A HPTLC Densitometer Determination of Sinapic acid in Chandrasur (Lepidium sativum)

Authors

  • Preeti Sagar Nayak JAWAHARLAL NEHRU KRISHI VISHWA VIDYALAYA
  • Sunil D Upadhyaya JAWAHARLAL NEHRU KRISHI VISHWA VIDYALAYA
  • Anubha Upadhyaya JAWAHARLAL NEHRU KRISHI VISHWA VIDYALAYA

DOI:

https://doi.org/10.3329/jsr.v1i1.1196

Keywords:

Lepidium sativum, Sinapic acid, HPTLC, Photo densitometry

Abstract

A qualitative and quantitative analysis method was established to improve quality assessment standards for Lepidium sativum seeds. The sinapic acid was identified by comparing with standards and quantified simultaneously by High performance thin layer chromatographic (HPTLC). HPTLC of Lepidium sativum methanolic extract was performed on Silica gel 60F254 [20 cm à 10 cm] plates with butanol: acetic acid: water (4:1:5), as mobile phase. Quantitative evaluation of the plate was performed in the absorbance-reflectance mode at 326 nm. The sinapic acid was separated on a thin layer of silica gel and determined by HPTLC-photo densitometry. The proposed method is simple, sensitive and can be used for the routine assay.

 

Keywords: Lepidium sativum; Sinapic acid; HPTLC; Photo densitometry.

 

© 2009 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights

reserved.

 

DOI: 10.3329/jsr.v1i1.1196

 

 

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Author Biographies

Preeti Sagar Nayak, JAWAHARLAL NEHRU KRISHI VISHWA VIDYALAYA

SENIOR RESEARCH FELLOW
DEPARTMENT OF CROP AND HERBAL PHYSIOLOGY,
COLLEGE OF AGRICULTURE,
JAWAHARLAL NEHRU KRISHI VISHWA VIDYALAYA
JNKVV - ADHARTAL
JABALPUR,
MADHYA PRADESH
INDIA 482004

Sunil D Upadhyaya, JAWAHARLAL NEHRU KRISHI VISHWA VIDYALAYA

PROFESSOR
DEPARTMENT OF CROP AND HERBAL PHYSIOLOGY,
COLLEGE OF AGRICULTURE,
JAWAHARLAL NEHRU KRISHI VISHWA VIDYALAYA
JNKVV - ADHARTAL
JABALPUR,
MADHYA PRADESH
INDIA 482004

Anubha Upadhyaya, JAWAHARLAL NEHRU KRISHI VISHWA VIDYALAYA

ASSOCIATE PROFESSOR
DEPARTMENT OF CROP AND HERBAL PHYSIOLOGY,
COLLEGE OF AGRICULTURE,
JAWAHARLAL NEHRU KRISHI VISHWA VIDYALAYA
JNKVV - ADHARTAL
JABALPUR,
MADHYA PRADESH
INDIA 482004

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Published

2008-12-25

How to Cite

Nayak, P. S., Upadhyaya, S. D., & Upadhyaya, A. (2008). A HPTLC Densitometer Determination of Sinapic acid in Chandrasur (Lepidium sativum). Journal of Scientific Research, 1(1), 121–127. https://doi.org/10.3329/jsr.v1i1.1196

Issue

Section

Section B: Chemical and Biological Sciences