Development and Validation of a RP-HPLC Method for the Quantification of Omeprazole in Pharmaceutical Dosage Form

Authors

  • R. Kayesh Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Dhaka, Dhaka-1000
  • M. Z. Sultan University of Dhaka, Dhaka-1000
  • A. Rahman Drug Analysis and Research Laboratory, Centre for Advanced Research in Sciences, University of Dhaka, Dhaka-1000
  • M. G. Uddin Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Dhaka, Dhaka-1000
  • F. Aktar Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Dhaka, Dhaka-1000
  • M. A. Rashid Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Dhaka, Dhaka-1000

DOI:

https://doi.org/10.3329/jsr.v5i2.12779

Keywords:

HPLC, Method development, Gradient condition, Validation, Omeprazole.

Abstract

A rapid and highly sensitive reversed phase high performance liquid chromatographic method has been developed for quantitative estimation of omeprazole in pharmaceutical preparations. The method has been validated according to FDA and USP guidelines with respect to accuracy, precision, specificity and linearity. The method was developed by using a gradient condition of mobile phase comprising 90% aqueous acetonitrile to 100% acetonitrile for 10 minutes at a flow rate of 0.7 mL/min over C-18 (ODS, 250 x 4.6 mm) column at ambient temperature. More than 97% recovery demonstrated the accuracy of the protocol. Intra-day and inter-day precision studies of the new method were less than the maximum allowable limit (RSD% £ 2.0 according to FDA). The method showed linear response with correlation coefficient (r2) value of 0.998.  Therefore, it was found to be accurate, reproducible, sensitive and less time consuming and can be successfully applied for the assay of omeprazole formulations.

Keywords: HPLC; Method development; Gradient condition; Validation; Omeprazole.

© 2013 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.

doi: http://dx.doi.org/10.3329/jsr.v5i2.12779        J. Sci. Res. 5 (2), 335-342  (2013)

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Author Biography

M. Z. Sultan, University of Dhaka, Dhaka-1000

Sr. Lecturer and Head, Department of Pharmacy

Drug Analysis and Research Laboratory, Centre for Advanced Research in Sciences

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Published

2013-04-22

How to Cite

Kayesh, R., Sultan, M. Z., Rahman, A., Uddin, M. G., Aktar, F., & Rashid, M. A. (2013). Development and Validation of a RP-HPLC Method for the Quantification of Omeprazole in Pharmaceutical Dosage Form. Journal of Scientific Research, 5(2), 335–342. https://doi.org/10.3329/jsr.v5i2.12779

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Section

Section B: Chemical and Biological Sciences