DNA Damage in Lymphocytes in Hypertensive Subjects in Bangladesh

Authors

  • M. Saiedullah Bangladesh Institute of Health Sciences (BIHS), & Bangladesh University of Health Sciences (BUHS)
  • S. Hayat Bangladesh Institute of Health Sciences (BIHS), & Bangladesh University of Health Sciences (BUHS)
  • M. R. Zamir Bangladesh University of Health Sciences (BUHS)
  • M. Arif University of Dhaka
  • Z. H. Howlader University of Dhaka
  • M. Rahman University of Dhaka

DOI:

https://doi.org/10.3329/jsr.v5i3.15022

Keywords:

Superoxide dismutase, Protein carbonyl content, Oxidative stress, Oxidative DNA damage, Hypertension, Bangladeshi population.

Abstract

Oxidative stress due to imbalance between the production of reactive oxygen species and their dismutation is claimed to be higher in hypertensive subjects than normotensive subjects. In hypertensive subjects oxidative stress may damage deoxy-ribonucleic acids (DNA). In this study plasma superoxide dismutase (SOD) activities, protein carbonyl contents (PCCs) and extent of DNA damage in lymphocytes were measured in specimens obtained from 86 subjects to compare oxidative stress and oxidative DNA damage between normotensive and hypertensive subjects and to assess their relationship with the degree of blood pressure. Results were expressed as mean±SD. Two-tailed unpaired t test and Pearsons correlation test were done to compare or to determine the relationship between groups or variables. SOD activities were 2.85±0.12 unit/mg protein and 3.84±0.45 unit/mg protein (p<0.05) in hypertensive and normotensive groups respectively. PCCs were 4.77±0.36 nmol/mg protein and 3.75±0.23 nmol/mg protein in hypertensive and normotensive groups respectively. Olive tail moments (OTM) were 124.7±11.69 units and 108.9±9.27 units in hypertensive and normotensive groups respectively. The correlation coefficient of OTM was 0.3924 (p<0.05) for diastolic blood pressure and 0.3618 (p<0.05) for systolic blood pressure. Oxidative stress and DNA damage was higher in hypertensives than normotensives and DNA damage correlated positively with blood pressure.


Keywords: Superoxide dismutase, Protein carbonyl content, Oxidative stress, Oxidative DNA damage, Hypertension, Bangladeshi population.


© 2013 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.

doi: http://dx.doi.org/10.3329/jsr.v5i3.15022 J. Sci. Res. 5 (3), 535-543 (2013)

 

Downloads

Download data is not yet available.
Abstract
749
PDF
588

Author Biographies

M. Saiedullah, Bangladesh Institute of Health Sciences (BIHS), & Bangladesh University of Health Sciences (BUHS)

Senior Scientific Officer, Department of Clinical Biochemistry & Assistant Professor (cc), Department of Applied Laboratory Sciences

S. Hayat, Bangladesh Institute of Health Sciences (BIHS), & Bangladesh University of Health Sciences (BUHS)

Scientific Officer, Department of Clinical Biochemistry & Lecturer, Department of Applied Laboratory Sciences

M. R. Zamir, Bangladesh University of Health Sciences (BUHS)

Department of Chemistry, Assistant Professor

M. Arif, University of Dhaka

Department of Biochemistry & Molecular Biology, Associate Professor

Z. H. Howlader, University of Dhaka

Department of Biochemistry & Molecular Biology, Professor

M. Rahman, University of Dhaka

Department of Biochemistry & Molecular Biology, Professor

Downloads

Published

2013-08-29

How to Cite

Saiedullah, M., Hayat, S., Zamir, M. R., Arif, M., Howlader, Z. H., & Rahman, M. (2013). DNA Damage in Lymphocytes in Hypertensive Subjects in Bangladesh. Journal of Scientific Research, 5(3), 535–543. https://doi.org/10.3329/jsr.v5i3.15022

Issue

Section

Section B: Chemical and Biological Sciences