Preliminary Characterization of Melanin Isolated from Fruits and Seeds of <i>Nyctanthes arbor-tristis</i>

Authors

  • P. Kannan Vellore Institute of Technology University, Vellore, (TN), India
  • D. Ganjewala Vellore Institute of Technology University, Vellore, (TN), India

DOI:

https://doi.org/10.3329/jsr.v1i3.2005

Keywords:

Melanin, Nyctanthes arbor-tristis, UV-visible spectroscopy, IR-spectroscopy, Oxidant.

Abstract

Melanin from Nyctanthes arbor-tristis fruits and seeds was isolated and purified by alkaline extraction, acid hydrolysis and organic solvents. Each, fruit and seed yielded melanin 50 mg/100g tissue weight. The melanins of fruit and seeds were analyzed by UV-visible and infrared (IR) spectroscopy for characterization. The IR spectrum of fruit and seed melanins did not match with each other and the seed melanin could be distinguished by their sharp peat at 285 nm from the fruit melanin in IR spectrum. Two melanins have shown absorptions above and below 1600 cm-1 in their respective IR spectrum. The melanins thus characterized were tested for their stability after incubating at different temperatures and in presence of oxidants (KMnO4 and K2Cr2O7) and metal ions (Mg++ and Zn++) for a specified period. The stability of the melanin was assessed by measuring changes in the absorbance at 285 nm. The results revealed that the fruit melanin was more stable at 50 ºC while the seed melanin was quite stable at 25 ºC. However, their stability was markedly affected by oxidants and metal ions.

 

Keywords: Melanin; Nyctanthes arbor-tristis; UV-visible spectroscopy; IR-spectroscopy; Oxidant.

 

© 2009 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.

 

DOI: 10.3329/jsr.v1i3.2005               J. Sci. Res. 1 (3), 655-661 (2009)

 

 

Downloads

Download data is not yet available.
Abstract
843
PDF
1092

Author Biographies

P. Kannan, Vellore Institute of Technology University, Vellore, (TN), India

P. Kannan 

Stem Cell Center, Christian Medical College, Vellore-6320014, Tamil Nadu, India

 

 

D. Ganjewala, Vellore Institute of Technology University, Vellore, (TN), India

Senior Assistant Professor

Plant Biotechnology

School of Biotechnology, Chemical and Biomedical Engineering, Vellore Institute of Technology 

University, Vellore-632014, Tamil Nadu, India


 

Downloads

Published

2009-08-29

How to Cite

Kannan, P., & Ganjewala, D. (2009). Preliminary Characterization of Melanin Isolated from Fruits and Seeds of <i>Nyctanthes arbor-tristis</i>. Journal of Scientific Research, 1(3), 655–661. https://doi.org/10.3329/jsr.v1i3.2005

Issue

Section

Section B: Chemical and Biological Sciences