A Feasibility Study for Assessment of In-situ Bioremediation Potential of a Crude Oil Degrading Pseudomonas Consortium

Authors

  • A. Mittal Department of Microbiology, Gurukul Kangri University, Haridwar, Uttarakhand, India
  • P. Singh Department of Microbiology, Gurukul Kangri University, Haridwar, Uttarakhand, India

Keywords:

Hydrocarbons, Pseudomonas, Biodegradation, Gas Chromatogram.

Abstract

A microcosm study evaluating inoculums addition of mixed bacterial consortium to stimulate in-situ bioremediation of crude oil contaminated soil was conducted. In feasibility study, out of five treatments the application of bacterial consortium, nutrients and environmental factors resulted in 79.16% removal of TPH in 60 days, compared to 30.24% removal of TPH carried out by indigenous microflora. Gas chromatograms of original spilled oil and bioremediated oil by the addition of developed consortium shows that Pr/Ph ratio decreased progressively from 2.358 to 1.626. The results showed that the ratio of di/tri aromatics decreased from initial 0.63 to 0.25 with progressive treatment of nutrient addition, as nutrient + tilling, nutrient + tilling + microbial seeding. Similar effect was observed in di/di + tri aromatics ratios which also decreased 0.31 to 0.20 by bioaugmentation only.

 

Keywords: Hydrocarbons; Pseudomonas; Biodegradation; Gas Chromatogram.

 

© 2010 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.

 

DOI: 10.3329/jsr.v2i1.2601                 J. Sci. Res. 2 (1), 127-137 (2010)

 

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Author Biographies

A. Mittal, Department of Microbiology, Gurukul Kangri University, Haridwar, Uttarakhand, India

Anupama Mittal

Department of Microbiology, Gurukul Kangri University, Haridwar, Uttarakhand, India

 

*Corresponding author. E-mail: anupma.garg@yahoo.com

Tel: +919999882926

 

P. Singh, Department of Microbiology, Gurukul Kangri University, Haridwar, Uttarakhand, India

Padma Singh

Department of Microbiology, Gurukul Kangri University, Haridwar, Uttarakhand, India

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Published

2009-12-29

How to Cite

A Feasibility Study for Assessment of In-situ Bioremediation Potential of a Crude Oil Degrading Pseudomonas Consortium. (2009). Journal of Scientific Research, 2(1), 127-137. https://doi.org/10.3329/jsr.v2i1.2601

Issue

Vol. 2 No. 1 (2010)

Section

Section B: Chemical and Biological Sciences

License

© Journal of Scientific Research

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Articles published in the "Journal of Scientific Research" are Open Access articles under a Creative Commons Attribution-ShareAlike 4.0 International license (CC BY-SA 4.0). This license permits use, distribution and reproduction in any medium, provided the original work is properly cited and initial publication in this journal. In addition to that, users must provide a link to the license, indicate if changes are made and distribute using the same license as original if the original content has been remixed, transformed or built upon.

How to Cite

A Feasibility Study for Assessment of In-situ Bioremediation Potential of a Crude Oil Degrading Pseudomonas Consortium. (2009). Journal of Scientific Research, 2(1), 127-137. https://doi.org/10.3329/jsr.v2i1.2601