A Feasibility Study for Assessment of <i>In-situ</i> Bioremediation Potential of a Crude Oil Degrading <i>Pseudomonas</i> Consortium

Authors

  • A. Mittal Department of Microbiology, Gurukul Kangri University, Haridwar, Uttarakhand, India
  • P. Singh Department of Microbiology, Gurukul Kangri University, Haridwar, Uttarakhand, India

DOI:

https://doi.org/10.3329/jsr.v2i1.2601

Keywords:

Hydrocarbons, Pseudomonas, Biodegradation, Gas Chromatogram.

Abstract

A microcosm study evaluating inoculums addition of mixed bacterial consortium to stimulate in-situ bioremediation of crude oil contaminated soil was conducted. In feasibility study, out of five treatments the application of bacterial consortium, nutrients and environmental factors resulted in 79.16% removal of TPH in 60 days, compared to 30.24% removal of TPH carried out by indigenous microflora. Gas chromatograms of original spilled oil and bioremediated oil by the addition of developed consortium shows that Pr/Ph ratio decreased progressively from 2.358 to 1.626. The results showed that the ratio of di/tri aromatics decreased from initial 0.63 to 0.25 with progressive treatment of nutrient addition, as nutrient + tilling, nutrient + tilling + microbial seeding. Similar effect was observed in di/di + tri aromatics ratios which also decreased 0.31 to 0.20 by bioaugmentation only.

 

Keywords: Hydrocarbons; Pseudomonas; Biodegradation; Gas Chromatogram.

 

© 2010 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.

 

DOI: 10.3329/jsr.v2i1.2601                 J. Sci. Res. 2 (1), 127-137 (2010)

 

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Author Biographies

A. Mittal, Department of Microbiology, Gurukul Kangri University, Haridwar, Uttarakhand, India

Anupama Mittal

Department of Microbiology, Gurukul Kangri University, Haridwar, Uttarakhand, India

 

*Corresponding author. E-mail: anupma.garg@yahoo.com

Tel: +919999882926

 

P. Singh, Department of Microbiology, Gurukul Kangri University, Haridwar, Uttarakhand, India

Padma Singh

Department of Microbiology, Gurukul Kangri University, Haridwar, Uttarakhand, India

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Published

2009-12-29

How to Cite

Mittal, A., & Singh, P. (2009). A Feasibility Study for Assessment of <i>In-situ</i> Bioremediation Potential of a Crude Oil Degrading <i>Pseudomonas</i> Consortium. Journal of Scientific Research, 2(1), 127–137. https://doi.org/10.3329/jsr.v2i1.2601

Issue

Section

Section B: Chemical and Biological Sciences