Antioxidant, Antibacterial and Cytotoxic Activity of the Methanol Extract of <i>Urtica Crenulata</i>

Authors

  • M. A. Rahman University of Chittagong
  • M. S. Rana Department of Biochemistry and Molecular Biology, University of Chittagong
  • M. M. Zaman Bangladesh Council for Scientific and Industrial Research (BCSIR), Chittagong
  • S. A. Uddin Institute of marine Science and Fisheries, University of Chittagong
  • R. Akter Bangladesh Council for Scientific and Industrial Research (BCSIR)

DOI:

https://doi.org/10.3329/jsr.v2i1.2872

Keywords:

Urtica crenulata, Antioxidant, antibacterial, Cytotoxic, BHT.

Abstract

The antioxidant, antibacterial and cytotoxic properties of methanol extract of Urtica crenulata (syn: Laportea crenulata Gaud) stem has been investigated in the present study. The antioxidant and cytotoxic activity of the Urtica crenulata methanol extract were assessed by DPPH free radical scavenging method and brine shrimp lethality bioassay method, respectively. The antibacterial activity of the plant extract (500 μg/disc) was also carried out by disc diffusion technique. Stem extract showed DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging effect compared with ascorbic acid. IC50 value of ascorbic acid and stem extract was found 14.72 μg/ml and 1468.9 μg/ml, respectively. In antibacterial experiment, Urtica crenulata stem extract showed 8, 14 and 10 mm of diameter of zone inhibition against Salmonella typhi, Shigella flexneri and Shigella sonnei, respectively and 9 and 8 mm of diameter of zone inhibition against Bacillus subtilis and Bacillus cereus but no activity was observed against Staphylococcus aureus. In brine shrimp lethality assay, the LC50 value of the extract was found 104.0 μg/ml, which indicates that the extract has high cytoxic effect. The present study demonstrates that methanol extract of Urtica crenulata stem has significant cytotoxic effect. The extract also showed some moderate antibacterial and minimum significant antioxidant effects.

 

Keywords: Urtica crenulata;  Antioxidant; Antibacterial; Cytotoxic; BHT.

 

© 2010 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.

 

DOI: 10.3329/jsr.v2i1.2872             J. Sci. Res. 2 (1), 169-177 (2010)

 

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Author Biographies

M. A. Rahman, University of Chittagong

Assistant Professor

M. S. Rana, Department of Biochemistry and Molecular Biology, University of Chittagong

Department of Biochemistry and Molecular Biology

M. M. Zaman, Bangladesh Council for Scientific and Industrial Research (BCSIR), Chittagong

Scientific Officer, BCSIR, Chittagong

S. A. Uddin, Institute of marine Science and Fisheries, University of Chittagong

Assistant Professor

R. Akter, Bangladesh Council for Scientific and Industrial Research (BCSIR)

Scientific Officer

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Published

2009-12-29

How to Cite

Rahman, M. A., Rana, M. S., Zaman, M. M., Uddin, S. A., & Akter, R. (2009). Antioxidant, Antibacterial and Cytotoxic Activity of the Methanol Extract of <i>Urtica Crenulata</i>. Journal of Scientific Research, 2(1), 169–177. https://doi.org/10.3329/jsr.v2i1.2872

Issue

Section

Section B: Chemical and Biological Sciences