Conversion of Waste Agriculture Biomass to Bioethanol by Recombinant <i>Saccharomyces cerevisiae</i>

Authors

  • A. A. Saleh Faculty of Engineering, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak
  • S. Hamdan Faculty of Engineering, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia
  • N. Annaluru John Hopkins Medical Institutions, Baltimore, MD 21205, USA
  • S. Watanabe Institute of Advanced Energy, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan
  • M. R. Rahman Faculty of Engineering, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak
  • T. Kodaki Institute of Advanced Energy, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan
  • K. Makino Institute of Advanced Energy, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan

DOI:

https://doi.org/10.3329/jsr.v2i2.2882

Keywords:

Agricultural waste biomass, Protein engineering, Xylitol dehydrogenase, Xylose-fermentation, Ethanol production.

Abstract

Agricultural waste biomass has already been transferred to bioethanol and used as energy related products, although many issues such as efficiency and productivity still to be overcome. In this study, the protein engineering was applied to generate enzymes with completely reversed coenzyme specificity and developed recombinant yeasts containing those engineered enzymes for construction of an efficient biomass-ethanol conversion system. Recombinant yeasts were constructed with the genes encoding a wild type xylose reductase (XR) and the protein engineered xylitol dehydrogenase (XDH) (with NADP) of Pichia stipitis.  These recombinant yeasts were characterized based on the enzyme activity and fermentation ability of xylose to ethanol. The protein engineered enzymes were expressed significantly in Saccharomyces cerevisiae as judged by the enzyme activity in vitro. Ethanol fermentation was measured in batch culture under anaerobic conditions. The significant enhancement was found in Y-ARS strain, in which NADP+-dependent XDH was expressed; 85% decrease of unfavorable xylitol excretion with 26% increased ethanol production, when compared with the reference strain expressing the wild-type XDH.

 

Keywords: Agricultural waste biomass; Protein engineering; Xylitol dehydrogenase; Xylose-fermentation; Eethanol production.

 

© 2010 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.

 

DOI: 10.3329/jsr.v2i2.2882               J. Sci. Res. 2 (2), 351-361 (2010)

 

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Author Biographies

A. A. Saleh, Faculty of Engineering, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak

Faculty of Engineering, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia

S. Hamdan, Faculty of Engineering, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia

Sinin Hamdan

 

Faculty of Engineering, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia

N. Annaluru, John Hopkins Medical Institutions, Baltimore, MD 21205, USA

Narayana Annaluru

John Hopkins Medical Institutions, Baltimore, MD 21205, USA

 

S. Watanabe, Institute of Advanced Energy, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan

Seiya Watanabe

 

Institute of Advanced Energy, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan

M. R. Rahman, Faculty of Engineering, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak

Faculty of Engineering, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia

T. Kodaki, Institute of Advanced Energy, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan

Tsutomu Kodaki

Institute of Advanced Energy, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan

K. Makino, Institute of Advanced Energy, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan

Keisuke Makino

 

Institute of Advanced Energy, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan

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Published

2010-04-26

How to Cite

Saleh, A. A., Hamdan, S., Annaluru, N., Watanabe, S., Rahman, M. R., Kodaki, T., & Makino, K. (2010). Conversion of Waste Agriculture Biomass to Bioethanol by Recombinant <i>Saccharomyces cerevisiae</i>. Journal of Scientific Research, 2(2), 351–361. https://doi.org/10.3329/jsr.v2i2.2882

Issue

Section

Section B: Chemical and Biological Sciences