Protein Profiling of <i>Thiobacillus ferrooxidans</i> and <i>Pseudomonas fluorescens</i> Mutants during Metal Extraction

Authors

  • S. M. Shaikh Department of Botany, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad (MS) 431004
  • R. U. Shaikh
  • A. B. Ade

DOI:

https://doi.org/10.3329/jsr.v10i1.33641

Keywords:

Protein Profile, Metal extraction, Thiobacillus, Pseudomonas, Microbes, Mineral ore, Industrial waste.

Abstract

Proteins are specific for the specific task associated with the cell. The metal extracting bacteria, Thiobacillus ferrooxidans and Pseudomonas fluorescens have two different mechanisms for the metal extraction. One extracts the metals by donating electrons and by doing self-oxidation and another is accumulating the metals onto its cell surface which is negatively charged. Therefore to differentiate the task of metal extraction protein profiling was done and compared. The water soluble proteins were analyzed through SDS-PAGE. There was no significant difference in the profiles of both.

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Author Biography

S. M. Shaikh, Department of Botany, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad (MS) 431004

Botany

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Published

2018-01-01

How to Cite

Shaikh, S. M., Shaikh, R. U., & Ade, A. B. (2018). Protein Profiling of <i>Thiobacillus ferrooxidans</i> and <i>Pseudomonas fluorescens</i> Mutants during Metal Extraction. Journal of Scientific Research, 10(1), 61–66. https://doi.org/10.3329/jsr.v10i1.33641

Issue

Vol. 10 No. 1 (2018)

Section

Section B: Chemical and Biological Sciences

License

© Journal of Scientific Research

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Articles published in the "Journal of Scientific Research" are Open Access articles under a Creative Commons Attribution-ShareAlike 4.0 International license (CC BY-SA 4.0). This license permits use, distribution and reproduction in any medium, provided the original work is properly cited and initial publication in this journal. In addition to that, users must provide a link to the license, indicate if changes are made and distribute using the same license as original if the original content has been remixed, transformed or built upon.