Screening of Extracellular Keratinase Producing Bacteria from Feather Processing Areas in Vellore, Tamil Nadu, India

Authors

  • H. Prasad V VIT University, Vellore,T.N
  • G. Kumar VIT Unibrtdity,Vellore, T.N.
  • Kartik L
  • B. Rao KV VIT University, Vellotre-14, T.N., India

DOI:

https://doi.org/10.3329/jsr.v2i3.4567

Keywords:

Keratinolytic, Spread plate method, Bacillus sp., Bacteria.

Abstract

The aim of the current study was to isolate keratinolytic bacteria from the soil samples collected from different feather processing areas in Vellore, TN, India. The isolation was performed by serial dilution and plating method. Total eight bacteria were isolated from the collected soil samples. All isolates were screened for keratinolytic activity by Casein agar plate method, among eight bacterial isolates only one (H5) isolate showed the keratinolytic activity in Casein agar medium. H5 isolate (potential strain) was identified as Bacillus sp. by microscopic and biochemical experiments. The best enzyme activity was observed at pH 7 and temperature 30ºC.

 

Keywords: Keratinolytic; Spread plate method; Bacillus sp.; Bacteria.

 

© 2010 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.

 

DOI: 10.3329/jsr.v2i3.4567              J. Sci. Res. 2 (3), 559-565 (2010)

 

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Author Biographies

H. Prasad V, VIT University, Vellore,T.N

M.Sc. Student, School of Bioscience and Technology, VIT University, Vellore, Tamil Nadu-632 014, India

 

G. Kumar, VIT Unibrtdity,Vellore, T.N.

Research Scholar, School of Bioscience and Technology, VIT University, Vellore, Tamil Nadu-632 014, India

 

Kartik L

Research Scholar, School of Bioscience and Technology, VIT University, Vellore, Tamil Nadu-632 014, India

 

B. Rao KV, VIT University, Vellotre-14, T.N., India

Asst. Prof.(Sr.)

School of Bioscience and Technology, VIT University, Vellotre-14, T.N., India

 

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Published

2010-08-24

How to Cite

Prasad V, H., Kumar, G., L, K., & KV, B. R. (2010). Screening of Extracellular Keratinase Producing Bacteria from Feather Processing Areas in Vellore, Tamil Nadu, India. Journal of Scientific Research, 2(3), 559. https://doi.org/10.3329/jsr.v2i3.4567

Issue

Section

Section B: Chemical and Biological Sciences