Optimization of Cultural Conditions for Keratinase Production by <i>Curvularia lunata</i> (JK17) using Response Surface Methodology

Authors

  • Meenakshi Department of Botany, Dolphin PG College of Science and Agriculture, Chunni kalan Fatehgarh Sahib (Punjab) India 140407
  • J. Kumar Department of Botany, Dolphin PG College of Science and Agriculture, Chunni kalan Fatehgarh Sahib (Punjab) India 140407; Department of Biosciences, UIBT Chandigarh University Mohali (Punjab) India 140413 https://orcid.org/0000-0002-5861-8562
  • R. Yadav Indian Plywood Industries Research and Training Institute Centre Mohali, (Punjab) India 160055

DOI:

https://doi.org/10.3329/jsr.v14i1.53950

Abstract

This study presents optimum cultural conditions for the maximum keratinase production in SSF applying response surface methodology. The maximum enzyme activity of 123 U/mL for Curvularia lunata was observed at cultural condition 12.72 days incubation, temp. 35oC, 5.6 g feather, 4.9 g carbon, 5.9 g nitrogen. Three-dimensional response surface and contour plots were drawn to find the relationship between keratinase production and cultural conditions. These values optimum for keratinase production by Curvularia lunata were in good accord with the experimental result.

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Author Biography

J. Kumar, Department of Botany, Dolphin PG College of Science and Agriculture, Chunni kalan Fatehgarh Sahib (Punjab) India 140407; Department of Biosciences, UIBT Chandigarh University Mohali (Punjab) India 140413

 

 

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Published

2022-01-01

How to Cite

Meenakshi, Kumar, J., & Yadav, R. (2022). Optimization of Cultural Conditions for Keratinase Production by <i>Curvularia lunata</i> (JK17) using Response Surface Methodology. Journal of Scientific Research, 14(1), 363–374. https://doi.org/10.3329/jsr.v14i1.53950

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Section

Section B: Chemical and Biological Sciences