Isolation and Screening of Thermo stable Extracellular Alkaline Protease Producing Bacteria from Tannery Effluents

Authors

  • S. M. A. Habib Department of Environmental Sciences, Jahangirnagar University, Savar, Dhaka 1342
  • A. N. M. Fakhruddin Department of Environmental Sciences, Jahangirnagar University, Savar, Dhaka 1342
  • S. Begum Department of Microbiology and Biotechnology, Jagannath University, Dhaka 1100
  • M. M. Ahmed Institute of Food Science and Technology, Bangladesh Council of Scientific and Industrial Research, Dhaka 1205

DOI:

https://doi.org/10.3329/jsr.v4i2.6976

Keywords:

Actinobacillus, Detergent, Halobacterium, Protease, Proteolytic activity, Tannery.

Abstract

The aim of the current study was to isolate extracellular protease producing bacteria from tannery effluents. The isolation was performed by serial dilution and plating method. Eight protease producing isolates were screened out on the basis of their clear zone formation on skim milk agar as well as production of protease in protease producing broth. They were identified on the basis of cultural, morphological and biochemical tests. Among the eight isolates, 3 isolates were found to belong to the genus Halobacterium, and the rest 5 isolates were found to belong to genus Actinobacillus. Among the eight protease producing isolates, one of the isolates was selected as promising protease producer and designated as Halobacterium sp. AF1.

Keywords: Actinobacillus; Detergent; Halobacterium; Protease; Proteolytic activity; Tannery.

© 2012 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.

doi: http://dx.doi.org/10.3329/jsr.v4i2.6976 J. Sci. Res. 4 (2), 515-522 (2012)

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Author Biographies

S. M. A. Habib, Department of Environmental Sciences, Jahangirnagar University, Savar, Dhaka 1342

MS student, Department of Environmental Sciences, Jahangirnagar University

A. N. M. Fakhruddin, Department of Environmental Sciences, Jahangirnagar University, Savar, Dhaka 1342

Associate Professor,

Department of Environmental Sciences, Jahangirnagar University

S. Begum, Department of Microbiology and Biotechnology, Jagannath University, Dhaka 1100

Assistant Professor, Department of Microbiology and Biotechnology

M. M. Ahmed, Institute of Food Science and Technology, Bangladesh Council of Scientific and Industrial Research, Dhaka 1205

Senior Scientific Officer, Institute of Food Science and Technology, Bangladesh Council of Scientific and Industrial Research, Dhaka 1205.

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Published

2012-04-27

How to Cite

Habib, S. M. A., Fakhruddin, A. N. M., Begum, S., & Ahmed, M. M. (2012). Isolation and Screening of Thermo stable Extracellular Alkaline Protease Producing Bacteria from Tannery Effluents. Journal of Scientific Research, 4(2), 515. https://doi.org/10.3329/jsr.v4i2.6976

Issue

Section

Section B: Chemical and Biological Sciences