Effect of Sodium Dodecyl Sulfate and Acridine Orange on Isolation of Plasmid and Antimicrobial Resistance Pattern of Clinical Isolates of <i>Klebsiella sp.</i>

Abstract

The susceptibility pattern of 25 Klebsiella sp. samples, collected for a six months time period from diagnostic centers, was investigated against 16 different antimicrobials. All the isolates of this study exhibit resistance against cephalexin, cephradine, ciporfloxacin, cloxacillin, erythromycin, oxacillin, rifampicin and tetracycline. Most of the isolates were resistant to amoxicillin (92%), vancomycin (96%), neomycin (84%) and chloramphenicol (76%). A few of the isolates showed resistance to tetracycline (36%), ceftriaxone (40%) and gentamycin (56%). Seven multidrug resistant (MDR) out of the 25 isolates were selected and grown in varying concentrations of sodium dodecyl sulfate (SDS) in Luria broth. Their plasmid profile was also analyzed. Distinct plasmid bands were observed when the MDR strains of Klebsiella sp. were grown in 5% SDS and acridine orange (10 µg/ml) in Luria broth. One of the isolates (isolate no. 3) treated with 5% SDS and/or 5% SDS along with acridine orange developed sensitivity against ceftriaxone, ciprofloxacin, co-trimoxazole, gentamycin and neomycin.

Keywords: Plasmid; Antimicrobial susceptibility; Multidrug resistant; Sodium dodecyl sulfate (SDS).

© 2012 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.

doi: http://dx.doi.org/10.3329/jsr.v4i2.8445 J. Sci. Res. 4 (2), 499-505 (2012)