Nanoencapsulation of Nisin and Ethylenediaminetetraacetic Acid in Niosomes and Their Antibacterial Activity

Authors

  • P. Kopermsub National Nanotechnology Center, National Science and Technology Development Agency, Klong 1, Klong Luang, Pathumthani, 12120
  • V. Mayen National Nanotechnology Center, National Science and Technology Development Agency, Klong 1, Klong Luang, Pathumthani, 12120, Thailand
  • C. Warin National Nanotechnology Center, National Science and Technology Development Agency, Klong 1, Klong Luang, Pathumthani, 12120, Thailand

DOI:

https://doi.org/10.3329/jsr.v4i2.9409

Keywords:

antibacterial, EDTA, Escherichia coli, niosomes, nisin, Staphylococcus aureus

Abstract

Ethylenediaminetetraacetic acid or EDTA was used complementarily with nisin to give a synergistic antibacterial effect against Gram positive and negative bacteria. Nisin and EDTA were encapsulated in nonionic surfactant vesicles or niosomes. Sorbitan monooleate and polyethylene glycol were precursors in preparation of niosomes. Size reduction of niosomes was conducted via extrusion through polycarbonate membrane with pore size of 200 nm. Diameters of prepared blank niosomes and nisin-EDTA-encapsulated niosomes were approximately 130 nm and 270 nm, respectively. Bilayer structure of niosomes was observed from negative staining-transmission electron microscopic images. Long-termed investigation of antimicrobial activity of nisin-EDTA-encapsulated niosomes and free nisin/EDTA were conducted against Staphylococcus aureus and Escherichia coli. Bacterial counts denoted a slow release of nisin-EDTA-encapsulated niosomes overtime whilst free nisin/EDTA gave a sudden bactericidal activity. Due to that free nisin/EDTA was immediately exploited at the beginning, bacterial counts then tended towards higher during the latter time of antimicrobial activity test.

 

Keywords: antibacterial; EDTA; Escherichia coli; niosomes; nisin; Staphylococcus aureus.

© 2012 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.

doi: http://dx.doi.org/10.3329/jsr.v4i2.9407 J. Sci. Res. 4 (2), 457-465 (2012)

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Published

2012-04-27

How to Cite

Kopermsub, P., Mayen, V., & Warin, C. (2012). Nanoencapsulation of Nisin and Ethylenediaminetetraacetic Acid in Niosomes and Their Antibacterial Activity. Journal of Scientific Research, 4(2), 457. https://doi.org/10.3329/jsr.v4i2.9409

Issue

Vol. 4 No. 2 (2012)

Section

Section B: Chemical and Biological Sciences

License

© Journal of Scientific Research

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Articles published in the "Journal of Scientific Research" are Open Access articles under a Creative Commons Attribution-ShareAlike 4.0 International license (CC BY-SA 4.0). This license permits use, distribution and reproduction in any medium, provided the original work is properly cited and initial publication in this journal. In addition to that, users must provide a link to the license, indicate if changes are made and distribute using the same license as original if the original content has been remixed, transformed or built upon.