Isolation and characterization of arsenic resistant soil bacteria and their effects on germination of rice under arsenic contamination

Authors

  • Istiaq Ahmed Department of Agricultural Chemistry, Faculty of Agriculture, Bangladesh Agricultural University, Mymensingh-2202
  • Md Tofazzal Islam Department of Biotechnology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Salna, Gazipur
  • Md Akhter Hossain Chowdhury Department of Agricultural Chemistry, Faculty of Agriculture, Bangladesh Agricultural University, Mymensingh-2202
  • Md Kamruzzaman Plant Breeding Division, Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh-2202

DOI:

https://doi.org/10.3329/ralf.v2i2.25003

Keywords:

Arsenic, Rice, Bacteria, Resistant, Germination, Growth

Abstract

This study was carried out to isolate, screen and characterize arsenic (As) resistant bacteria from As contaminated soils of Dumrakandi and Matlab under Faridpur and Chandpur districts and to evaluate their efficiency in reducing As toxicity against rice seedlings during germination. Thirteen strains were isolated from the soils which showed resistance to different levels of sodium arsenite (viz. 5, 10, 20 and 40 mM) in both agar plate and broth assay using BSMY I media. Among the isolates, BTL0011, BTL0012, BTL0015 and BTL0022 showed highest resistance to 40 mM sodium arsenite. Gram staining and KOH solubility test revealed that five strains were gram positive and rest eight was gram negative. They grew well in the liquid media at pH 5.5 to 8.5. In-vitro rice seedling bioassay with two superior isolates (BTL0011 and BTL0022) revealed that As resistant strains significantly enhanced seed germination of BRRI dhan29 and BRRI dhan47 at 60 ppm As. This study was laid out in CRD with three replications. The performance of BTL 0022 was superior to BTL0011. The overall results suggest that BTL0011 and BTL0022 can be used for bioremediation of As contaminated soils and to increase the germination and seedling growth of rice in As contaminated soils.

Res. Agric., Livest. Fish.2(2): 229-237, August 2015

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Author Biography

Istiaq Ahmed, Department of Agricultural Chemistry, Faculty of Agriculture, Bangladesh Agricultural University, Mymensingh-2202



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Published

2015-09-16

How to Cite

Ahmed, I., Islam, M. T., Chowdhury, M. A. H., & Kamruzzaman, M. (2015). Isolation and characterization of arsenic resistant soil bacteria and their effects on germination of rice under arsenic contamination. Research in Agriculture Livestock and Fisheries, 2(2), 229–237. https://doi.org/10.3329/ralf.v2i2.25003

Issue

Vol. 2 No. 2 (2015)

Section

Agriculture

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