Comparative efficacy of two extenders on post-thaw sperm characteristics of cryopreserved Black Bengal buck semen
Keywords:Comparative efficacy, Motility, EYC, TRIS, Black Bengal buck
The research work was conducted at the Artificial Insemination (AI) Center under the Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh to compare the efficacy between Egg Yolk Citrate (EYC) and Tris extenders for cryopreserved semen production in Black Bengal buck. The parameters of semen characteristics included percentage of individual motility (progressive), normal and live sperm and sperm concentration (billion/ml). Statistical analysis of fresh semen of individual buck effect was found to be significant (p<0.01) on normal sperm percentage but insignificant on individual motility, sperm concentration and live sperm percentage. After dilution with EYC extender, individual bucks had significant effect (p<0.05) on sperm motility and but insignificant on motility after cooling with and without glycerol. In the same way with Tris extender, it was insignificant (p>0.05) on diluted semen motility and motility after cooling with and without glycerol. Motility and morphology of the sperm after equilibration and thawing showed insignificant difference among the bucks using EYC extender. On the other hand, variation in the motility after equilibration and thawing was found significant (p<0.05) using Tris extender and insignificant on normal and live sperm percentages.
Res. Agric., Livest. Fish.6(1): 119-125, April 2019
How to Cite
All RALF articles are published under the terms of the Creative Commons Attribution License 4.0 International License. Readers can copy, distribute, transmit and adapt the work provided the original work and source is appropriately cited.
Submission of a manuscript implies that authors have met the requirements of the editorial policy and publication ethics. Authors retain the copyright of their articles published in the journal. However, authors agree that their articles remain permanently open access under the terms of the Creative Commons Attribution License 4.0 International License.