Synergistic Effects of Vitamin A and Spirulina on Arsenic Load in Rat Tissues and Blood

Authors

  • Md Ashraful Islam College of Veterinary Medicine, Chungbuk National University, Cheongju, 28644, South Korea; and Department of Livestock services (DLS), Ministry of Fisheries and Livestock (MOFL), Dhaka, Bangladesh
  • Md Sodrul Islam Department of Physiology and Pharmacology, Faculty of Veterinary Medicine and Animal Science, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, Bangladesh
  • Md Abdul Awal Department of Pharmacology, Bangladesh Agricultural University, Mymensingh, Bangladesh
  • Md Zahorul Islam Department of Pharmacology, Bangladesh Agricultural University, Mymensingh, Bangladesh
  • A Khair Risk Management Consultant for Animal and Animal-Source Products. Bangladesh Trade Facilitation Project, Venture37, Land O’Lakes, Dhaka, Bangladesh
  • Mohammed Mebarek Bia Department of Parasitology, Parasitology Research Center and International Parasite Resource Bank, Chungbuk National University, School of Medicine, Cheongju 28644, Republic of Korea
  • Obaidul Islam College of Veterinary Medicine, Chungbuk National University, Cheongju, 28644, South Korea

DOI:

https://doi.org/10.3329/ralf.v10i3.71005

Keywords:

Arsenic, Vitamin A, Spirulina, Rats

Abstract

Arsenic (As) is found in contaminated groundwater as the source of pollution. In this study, 60 Long-Evans rats were used to assess the levels of As in the blood and organs and to compare the effectiveness of vitamin A and spirulina (Spirulina platensis) in preventing a chronic As accumulation. Twelve rats were assigned to each group of animals. The experimental groups were the control (T0), As (T1), As + spirulina (T2), As + vitamin A (T3), and As + spirulina + vitamin A (T4). The T1, T2, T3, and T4 groups were orally administered with sodium arsenite (NaAsO2) @ 4 mg/kg body weight (BW) for 63 days. In addition to NaAsO2, the T2 and T4 received 1 g/kg BW spirulina. The T3 and T4 received 2500 IU/kg BW vitamin A for 63 days, respectively. Four rats were euthanized in each group to evaluate the As concentration in the liver, lung, kidney, and blood at an interval of 21 days. Total As concentration was quantified from the organs using Hydride Generation Atomic Absorption Spectrophotometer (HG-AAS). The results revealed that the T0 had no visible clinical symptoms. However, after 63 days of treatment, the T1 (As only administration) accumulated more As compared to other groups. The concentration of As was highest in the blood, then in the kidney, liver, and lung. In this case, spirulina and vitamin A substantially (p<0.01) decreased the concentration of As in the rats' organs and tissues Spirulina is more effective than vitamin A in reducing As accumulation in rats. In summary, the combination of both spirulina and vitamin A has a positive impact on reducing the accumulation of chronic arsenicosis in rats compared to the individual administration of either spirulina or vitamin A alone.

Vol. 10, No. 3, December 2023: 341-351

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Published

2024-01-21

How to Cite

Md Ashraful Islam, Md Sodrul Islam, Md Abdul Awal, Md Zahorul Islam, A Khair, Mohammed Mebarek Bia, & Obaidul Islam. (2024). Synergistic Effects of Vitamin A and Spirulina on Arsenic Load in Rat Tissues and Blood. Research in Agriculture Livestock and Fisheries, 10(3), 341–351. https://doi.org/10.3329/ralf.v10i3.71005

Issue

Vol. 10 No. 3 (2023)

Section

Livestock

License

Copyright (c) 2023 Md Ashraful Islam, Md. Sodrul Islam, Md Abdul Awal, Md. Zahorul Islam, A. Khair, Mohammed Mebarek Bia, Obaidul Islam

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This work is licensed under a Creative Commons Attribution 4.0 International License.

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