Effects of anticoagulant (EDTA and Heparin) on blood of goat.
DOI:
https://doi.org/10.3329/ralf.v11i1.72915Keywords:
Anticoagulant, EDTA, Heparin, Blood, Hematology, GoatAbstract
Blood is an important tool for accurate diagnosis of disease, forensic investigation, and hematological analysis. However this phenomenon can be thwarted occasionally if appropriate anticoagulant with storage times is not maintained properly. This research aims to compare and explore the effect of anticoagulants (such as ethylene diamine tetra acetic acid and heparin) and storage time on the hematological parameters in indigenous goats. Twenty goats were enrolled and raised for this purpose. Bloods were collected into two different tubes containing EDTA and heparin and analyzed immediately to evaluate the basal value. All tubes were divided into two aliquots and stored at 4°C and 25°C and were analyzed again at the 24th and 48th hours of collection. The statistical analysis of this result showed that there was no significant difference (P>0.05) in the anticoagulant’s effect on the hematological parameters. RBC, Hb, PCV, MCV, MCH, MCHC, total WBC, and Platelets except ESR decreased gradually along with the storage time of up to 48 hours compared to the basal value. The hematological parameters were reduced more significantly (P<0.05) when stored at 250C rather than 4°C indicating that the parameters remain in better condition in refrigeration. The samples should be stored at 4°C rather than room temperature and be used within 24 hours because the storage time modify the analyzed results. These findings demonstrate that both of the anticoagulants (EDTA and Heparin) show reliable results, therefore it can be used to store blood samples for any diagnostic purpose.
Res. Agric. Livest. Fish. Vol. 11, No. 1, April 2024: 1-10
Downloads
84
172
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2024 Sushanto Chandro Roy, Sushanto Chandro Roy, Md Zamal Uddin, Sm Ahasanul Hamid, Ahasanul Hamid, Moizur Rahman, Md Royhan Gofur, S M Kamruzzaman
This work is licensed under a Creative Commons Attribution 4.0 International License.
Creative Commons
All RALF articles are published under the terms of the Creative Commons Attribution License 4.0 International License. Readers can copy, distribute, transmit and adapt the work provided the original work and source is appropriately cited.
Copyright
Submission of a manuscript implies that authors have met the requirements of the editorial policy and publication ethics. Authors retain the copyright of their articles published in the journal. However, authors agree that their articles remain permanently open access under the terms of the Creative Commons Attribution License 4.0 International License.