Efficacy of Terminalia arjuna (Roxb.) bark extracts against Salmonella sp. isolated from aquaculture ponds

Authors

  • Sohidul Islam Department of Aquaculture, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
  • Kartik Chandra Saha Department of Agricultural Chemistry, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
  • Naima Sultana
  • Md Raimur Rahman Department of Aquaculture, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
  • Johir Raihan Department of Aquaculture, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
  • Kaniz Farjana Department of Aquaculture, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
  • Rakiba Binta Raihan Department of Aquaculture, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
  • Tamanna Tabassum Department of Aquaculture, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
  • Sourav Acharjee Department of Aquaculture, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
  • Shadman Shakib Shovon Department of Aquaculture, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
  • Salman Shahriar Nibir Department of Aquaculture, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
  • Khadiza Tul Kubra Department of Aquaculture, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
  • Tanvir Rahman Department of Aquaculture, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

DOI:

https://doi.org/10.3329/ralf.v11i2.76076

Keywords:

Terminalia arjuna, Salmonella sp., Sensitivity, Drug resistance, Aquaculture

Abstract

The present study was conducted to determine the efficacy of Terminalia arjuna bark extracts against Salmonella sp. isolated from 15 aquaculture ponds located adjacent to Bangladesh Agricultural University, Mymensingh. T. arjuna barks was dried, powdered and extracted using cold water (polar) and hexane (non-polar) and stored at 10ºC while Salmonella sp. were isolated using SS (Salmonella Shigella) Agar and selected 2 isolates were confirmed by Hi Assorted biochemical test kit. Antimicrobial activities of the bark extract of T. arjuna against fifteen selected isolates of Salmonella were then determined. Two of those selected isolates (Sa-2 and Sa-5) shown zone of inhibition against crude bark extract (hexane extract) of T. arjuna. The isolates were also screened for antibiotic resistance to eleven antibiotics. Salmonella isolates were serotyped and the antimicrobial resistance was determined by a disk diffusion method. About 100% of the isolates were resistant to antibiotic discs among which ampicillin (10 μg), and chloramphenicol (30 μg) showed 15%, azithromycin (10 μg) showed 45%, erythromycin (15 μg) showed 60%, tetracycline (10 μg) showed 40%, ampicillin (10 μg) showed 100%, doxycycline (30 μg) showed 46.67%, kanamycin (30 μg) and gentamycin (10 μg) showed 6%, streptomycin (25 μg) and ciprofloxacin (10 μg)  showed 20%, and cefotaxime (30 μg) showed 30% resistant to isolated Salmonella sp. and all Salmonella sp. isolates were found to be  100% resistant to cold water extract but sensitive to hexane extract of T. arjuna. The study showed hexane extract of T. arjuna would be potential, ecofriendly and biodegradable herbal medicine to control multi drug resistant Salmonella sp. as well as aquaculture health management.

Res. Agric. Livest. Fish. Vol. 11, No. 2, August 2024: 255-269

 

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Published

2024-09-09

How to Cite

Islam, S., Saha, K. C., Sultana, N., Rahman, M. R., Raihan, J., Farjana, K., Raihan, R. B., Tabassum, T., Acharjee, S., Shovon, S. S., Nibir, S. S., Kubra, K. T., & Rahman, T. (2024). Efficacy of Terminalia arjuna (Roxb.) bark extracts against Salmonella sp. isolated from aquaculture ponds. Research in Agriculture Livestock and Fisheries, 11(2), 255–269. https://doi.org/10.3329/ralf.v11i2.76076

Issue

Vol. 11 No. 2 (2024)

Section

Fisheries

License

Copyright (c) 2024 Sohidul Islam, Mr Kartik Chandra Saha, Naima Sultana, Md Raimur Rahman, Johir Raihan, Kaniz Farjana, Rakiba Binta Raihan, Tamanna Tabassum, Sourav Acharjee, Shadman Shakib Shovon, Salman Shahriar Nibir, Khadiza Tul Kubra, Tanvir Rahman

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