Establishment of a Simple and Efficient Single Spore Isolation Method for Gilled Mushroom

Authors

  • Sharita Islam Department of Life Sciences, School of Environment and Life Sciences, Independent University Bangladesh, Dhaka, Bangladesh
  • Md Asif Hasan Mazumder Niloy Department of Life Sciences, School of Environment and Life Sciences, Independent University Bangladesh, Dhaka, Bangladesh
  • Sabina Yesmin Biotechnology Division, Bangladesh Agricultural Research Institute, Gazipur, Dhaka
  • Mahiea Hossain Mahi Department of Life Sciences, School of Environment and Life Sciences, Independent University Bangladesh, Dhaka, Bangladesh
  • Jebunnahar Khandakar Department of Life Sciences, School of Environment and Life Sciences, Independent University Bangladesh, Dhaka, Bangladesh

DOI:

https://doi.org/10.3329/ralf.v11i3.78896

Keywords:

Single spore, Cotton swab, Serial dilution, Genetic improvement, Mushroom

Abstract

Single-spore isolation is a fundamental technique for exploring genetic diversity in fungi, particularly in heterothallic species like Pleurotus ostreatus. This study outlines a method for isolating single spores from fruiting bodies, aimed at facilitating genetic improvement of mushrooms. The approach is especially suitable for the laboratories of mushrooms which are not necessarily well-funded, requiring only basic laboratory equipment. It is simple, resistant to contamination, and highly efficient. Under sterile conditions, spores were collected from mature mushroom fruiting bodies using a sterilized cotton swab and subjected to serial dilutions in distilled water. Each dilution was plated on Potato Dextrose Agar (PDA) and then incubated, producing a consistent source of genetically uniform mycelium for further study. Further, microscopic examinations revealed the presence of clamp connections, specialized structures that ensure genetic stability and facilitate cell division during hyphal growth. This method is broadly applicable, though fungi that do not germinate on artificial media may require an alternative approach.

Res. Agric. Livest. Fish. Vol. 11, No. 3, December 2024: 383-388

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Published

2025-01-07

How to Cite

Sharita Islam, Md Asif Hasan Mazumder Niloy, Sabina Yesmin, Mahiea Hossain Mahi, & Jebunnahar Khandakar. (2025). Establishment of a Simple and Efficient Single Spore Isolation Method for Gilled Mushroom . Research in Agriculture Livestock and Fisheries, 11(3), 383–388. https://doi.org/10.3329/ralf.v11i3.78896

Issue

Vol. 11 No. 3 (2024)

Section

Agriculture

License

Copyright (c) 2024 Sharita Islam, Md Asif Hasan Mazumder Niloy, Sabina Yesmin, Mahiea Hossain Mahi, Jebunnahar Khandakar

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This work is licensed under a Creative Commons Attribution 4.0 International License.

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