Multiple Sequence Alignment and Phylogenetic Analysis of Growth Differentiation Factor 9 Gene for Egg Production in Poultry Species

Authors

  • Pretty Rachuene School of Agricultural and Environmental Sciences, Department of Agricultural Economics and Animal Production, University of Limpopo, Private bag X1106, Sovenga 0727, Limpopo, South Africa
  • Kagisho M Molabe School of Agricultural and Environmental Sciences, Department of Agricultural Economics and Animal Production, University of Limpopo, Private bag X1106, Sovenga 0727, Limpopo, South Africa.
  • Thobela L Tyasi School of Agricultural and Environmental Sciences, Department of Agricultural Economics and Animal Production, University of Limpopo, Private bag X1106, Sovenga 0727, Limpopo, South Africa.

DOI:

https://doi.org/10.3329/ralf.v12i1.81475

Keywords:

Bioinformatic analysis, Evolutionary tree, DNA sequences

Abstract

Growth differentiation factor 9 gene (GDF9) is an oocyte-secreted factor which controls the functions of the ovarian cells, regulates the development and caliber of the egg. GDF9 DNA and protein sequences from NCBI were used to computationally investigate molecular genetic variation in poultry species. Results showed that percentage similarities of DNA and protein sequences ranged from 78.45 to 92.80% and 83.63 to 94.74%, respectively. The phylogenetic analysis results showed that chicken and turkey were closely related. The high percent similarities and close relatedness of poultry species show that GDF9 can be used as a candidate gene for improving egg production in poultry species.

Res. Agric. Livest. Fish. Vol. 12, No. 1, April 2025: 63-72

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Published

2025-05-14

How to Cite

Pretty Rachuene, Kagisho M Molabe, & Thobela L Tyasi. (2025). Multiple Sequence Alignment and Phylogenetic Analysis of Growth Differentiation Factor 9 Gene for Egg Production in Poultry Species. Research in Agriculture Livestock and Fisheries, 12(1), 63–72. https://doi.org/10.3329/ralf.v12i1.81475

Issue

Vol. 12 No. 1 (2025)

Section

Livestock

License

Copyright (c) 2025 Pretty Rachuene, Kagisho M Molabe, Thobela L Tyasi

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This work is licensed under a Creative Commons Attribution 4.0 International License.

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