Polymorphism of Sakini Chicken Population From Different Locations/Altitudes of Nepal Using Randomly Amplified Polymorphic Dna Markers

  • NA Gorkhali National Animal Breeding and Genetics Research Centre Nepal Agricultural Research Council, Kathmandu, Nepal
  • R Dhakal National Animal Breeding and Genetics Research Centre Nepal Agricultural Research Council, Kathmandu, Nepal
  • S Sapkota National Animal Breeding and Genetics Research Centre Nepal Agricultural Research Council, Kathmandu, Nepal
  • P Koirala National Animal Breeding and Genetics Research Centre Nepal Agricultural Research Council, Kathmandu, Nepal
  • BR Pokhrel National Animal Breeding and Genetics Research Centre Nepal Agricultural Research Council, Kathmandu, Nepal
  • MR Kolachhapati Agriculture and Forestry University, Chitwan, Nepal
  • N Bhattarai Agriculture and Forestry University, Chitwan, Nepal
Keywords: Sakini chicken, Genetic polymorphism, Polymorphic DNA

Abstract

A study was conducted to evaluate genetic polymorphism in three Sakini chicken populations using random amplification of polymorphic DNA (RAPD) with seven highly polymorphic primers. All populations showed polymorphism with these primers that generates 59 different bands with an average of 8.4 bands per primer with 78.6% polymorphism nature. Primer OPA-16 produced the highest number of polymorphism bands 47 % and the lowest number of bands was produced by the OPA-05 primer 24 %. Differences for genetic distance (D) among populations were significant (P<0.05). A consensus dendogram was therefore developed to show the phylogenetic relationship among the populations. The cluster pattern is well supported by the principle component analysis that also separates all three populations of Sakini chicken into six major groups. The results provide evidence of the applicability of RAPD to determining genetic relatedness within and among different poultry populations and in developing reproducible markers useful in evaluating individual variation in poultry.

SAARC J. Agri., 18(2): 115-124 (2020)

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Abstract
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Published
2021-01-04
How to Cite
Gorkhali, N., Dhakal, R., Sapkota, S., Koirala, P., Pokhrel, B., Kolachhapati, M., & Bhattarai, N. (2021). Polymorphism of Sakini Chicken Population From Different Locations/Altitudes of Nepal Using Randomly Amplified Polymorphic Dna Markers. SAARC Journal of Agriculture, 18(2), 115-124. https://doi.org/10.3329/sja.v18i2.51113
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Articles